| 东方伊萨酵母菌对偶氮染料酸性大红GR的脱色优化及特性研究 |
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| 引用本文： | 卢婧,余志晟,张洪勋.东方伊萨酵母菌对偶氮染料酸性大红GR的脱色优化及特性研究[J].安徽农业科学,2014(8):2233-2236. |
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| 作者姓名： | 卢婧 余志晟 张洪勋 |
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| 作者单位： | 中国科学院大学资源与环境学院,北京,100049;中国科学院大学资源与环境学院,北京,100049;中国科学院大学资源与环境学院,北京,100049 |
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| 基金项目： | 国家自然科学基金（20877098）;中国科学院重点部署项目（KZZD-EW-09-1）. |
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| 摘 要： | 目的]优化东方伊萨酵母菌(Issatchenkia orientalis YP-1)对偶氮染料酸性大红GR的脱色条件,初步阐明脱色机理。方法]研究该菌在不同温度、pH、接种量、染料起始浓度、碳源和氮源条件对酸性大红GR的脱色影响,并且对脱色降解功能酶进行定位。结果]该菌在16 h内对浓度为50~100 mg/L酸性大红GR的脱色率达96%,其机理主要为降解脱色。东方伊萨酵母菌YP-1的最佳接种量不应低于10%,最佳pH在4~8之间,最佳温度为28℃,(NH4)2SO4浓度在0.1%以上,葡萄糖浓度不应低于1%。脱色后染料的主体结构被破坏,发生降解反应。该菌主要脱色降解功能酶为胞内酶。结论]东方伊萨酵母菌对酸性大红GR的脱色效果显著,在染料废水处理中将具有良好的应用前景。
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| 关 键 词： | 酵母菌 东方伊萨酵母菌YP-1 偶氮染料 降解脱色 |
The Decolorization Optimization and Characteristics of an Azo Dye Acid Scarlet GR by Yeast Issatchenkia orientalis YP-1 |
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| Institution: | LU ,ling, YU Zhi-sheng et al ( College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049) |
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| Abstract: | Objective ] The study aimed to discuss the optimal conditions and preliminary decolorization mechanism for the decolorization and degradation of azo dye Acid Scarlet GR by yeast strain Issatchenkia orientalis YP-1. Method] The effect of different temperatures, pH, inoculation volumes, initial dye concentrations, carbon and nitrogen concentrations on decolorization with I. orientalis YP-1 was discussed in detail, as well as its preliminary decolorization mechanism. Result] This strain achieved a maximal decolorization （96%） for Acid Scarlet GR （50 -100 mg/L） after incubation for 16 h. To get such decolorization, the optimal inoculation volume of this strain was set not less than 10%, optimal pH of culture medium ranging from 4 to 9, and concentrations of （ NH4 ）2SO4 and glucose not lower than 0.1% and 1% , respectively. In addition, the results showed that the main structure of acid scarlet GR was destroyed after decolorization and the main functional decolorization enzyme was intracellular enzyme. Conclusion] The yeast strain lssatchenkia orientalis YP-1 has relatively strong decolorization capability on azo dye acid scarlet GR, which has a good prospect in the biodegradation of dye wastewater. |
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| Keywords: | Yeast lssatchenkia orientalis YP-1 Azo dye Decolorization and degradation |
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