分光光度法测定植酸含量 |
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引用本文: | 郝红英,周,芳,周彩荣等.分光光度法测定植酸含量[J].安徽农业科学,2014(7):1907+1975-1907,1975. |
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作者姓名: | 郝红英 周 芳 周彩荣等 |
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作者单位: | [1]黄河科技学院,河南郑州450063 [2]2,郑州大学,河南郑州450¨00l [3]郑州开普工程技术有限公司,河南郑州450001 |
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摘 要: | 目的]为了寻找适合于标准植酸的测定及微量测定的方法。方法]采用可见分光光度法,测定植酸的含量。结果]在标准曲线下,植酸浓度在0~32μg/ml范围内与吸光度呈良好线性关系,方程为:Y=-2.284 2X+0.384 1(R=0.997 3),平均回收率为97.35%,RSD为4.82%(n=4)。结论]该方法操作简便、快速、准确,可作为植酸的定量分析方法。
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关 键 词: | 植酸 含量 可见分光光度法 |
Determination on Phytic acid by Spectrophotometry |
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Institution: | HAO Hong-ying et al ( Huanghe Science and Technology College, Zhengzhou, Henan 450063 ) |
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Abstract: | Objective] The research aimed to find the method suited to the measurement of standard phytic acid and microdetermination. Method] A method for determination of phytic acid by speetrophotometry was studied. Result] The regression equation within the range of' the standard sample concentration in 0 -32 μg/ml was Y = -2. 284 2X + 0. 384 1 with a good linearity (R = 0. 997 3 ). The average recovery rate was 97.35 % and RSD was 4.82% (n = 4). Conclusion] The method was simple, rapid, accurate and suitable for the quality evaluation of phytic acid. |
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Keywords: | Phytic acid Content Spectrophotometry |
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