| “胃萎缩症”仿刺参幼体及亲参组织中病毒观察 |
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| 引用本文： | 邓欢,周遵春,韩家波.“胃萎缩症”仿刺参幼体及亲参组织中病毒观察[J].水产学报,2008,32(2):315-320. |
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| 作者姓名： | 邓欢 周遵春 韩家波 |
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| 作者单位： | 辽宁省海洋水产科学研究院,辽宁省应用海洋生物开放实验室,辽宁,大连,116023 |
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| 基金项目： | 国家海洋局海洋公益专项
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辽宁省科技计划
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辽宁省大连市科技计划 |
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| 摘 要： | 海参含有丰富的蛋白质和粘多糖,营养和药用价值极高.全世界海参约有1200种,在我国海域分布的有140多种,其中以黄、渤海的仿刺参(Apostichopus japonicus)品质最佳1].近几年,仿刺参(俗称"刺参")人工养殖已成为辽宁、山东沿海水产业重要的养殖品种之一和新的经济增长点.
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| 关 键 词： | 仿刺参 胃萎缩症 病毒 |
| 文章编号： | 1000-0615(2008)02-0315-06 |
| 收稿时间： | 2008/2/27 0:00:00 |
| 修稿时间： | 2007年2月14日 |
An observation on the pathogeny of stomach atrophy in sea cucumber Apostichopus japonicus (Selenka) larva by electron microscope |
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| DENG Huan,ZHOU Zunchun and HAN Jiabo.An observation on the pathogeny of stomach atrophy in sea cucumber Apostichopus japonicus (Selenka) larva by electron microscope[J].Journal of Fisheries of China,2008,32(2):315-320. |
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| Authors: | DENG Huan ZHOU Zunchun and HAN Jiabo |
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| Abstract: | From May to June in 2006, an epidemic disease named stomach atrophy syndrome in sea cucumber Apostichopus japonicus occurred widely in Liaoning area during the hatching period. The influence area was large and mortality was high and no efficient remedy to control the development and spread of this epidemic. T
his epidemic occurred mainly at 5 to 7th days after larvae hatching. With the increasing of spawn times, the infected time became ahead and the larvae were diseased after being hatched 1 to 2 days. The stomach of diseased larvae shrinked gradually and the stomach wall tended to thick, rough and distorted till the stomach became nearly small globosity. In order to understand the kinds of pathogeny, the A. japonicus larvae and their parents were detected by the observations of negative stained samples and ultrathin sections. The tissue homogenate was prepared from the A. japonicus larvae and the tissue
of their parents. The tissue samples were centrifuged (13 000 r·min-1, 8 min) twice after homogenizing, freezing and thawing. The supernatant solution wa
s dripped on to a copper net and stained with 2% PTA for 5min and observed with electron microscopy. The diseased animals were also fixed in 2.5% glutaraldehyde and 1% osmium tetroxide solution. Samples were dehydrated and transferred into Epon 812 resin and then cut. Sections were stained with lead citrate and uranyl acetate and observed. The observed results showed that amount of virus particles were observed on negative staining samples in the larvae homogenate, and most of them are approximately 75-200 nm in diameter. The virus particles were also found in the gonad, body wall, the alimentary canal and the respiratory trees of corresponding parents hatched the larvae. Farther examination of larvae by electron microscopic observation of ultrathin sections showed that the virus affected mainly the connective tissue cells and epithelial cells. The virions were approximately spherical or hexagonal with envelope, and the core structures with high electron densities in the envelope were observed. The gonad, the body wall, the intestines and the respiratory tree of paternal and maternal parents were also observed by negative staining. The results showed that this kind of virion located in all the tested organs and tissues. |
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| Keywords: | Apostichopus japonicus stomach atrophy virus |
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