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Evaluation of genetic diversity and relationships within an on-farm collection of Perilla frutescens (L.) Britt. using microsatellite markers |
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| Authors: | Yong-Jin Park Anupam Dixit Kyung-Ho Ma Ju-Kyung Lee Myoung-Hee Lee Chan-Sik Chung Miyuki Nitta Kazutoshi Okuno Tae-San Kim Eun-Gi Cho V Ramanatha Rao |
| Institution: | 1. National Institute of Agricultural Biotechnology, Suwon, 441-707, Republic of Korea 2. Bioversity International?-?APO Regional Office, P.O. Box 236, UPM Post Office, Serdang, Selangor D.E., 43400, Malaysia 3. Plant Biotechnology Program, Division of Biotechnology, Kangwon National University, Chuncheon, 200-701, Republic of Korea 4. Yeongnam Agricultural Research Institute, Milyang, 627-803, Republic of Korea 5. National Institute of Agricultural Science (NIAS), Tsukuba, Ibaraki, 305-8602, Japan 6. Laboratory of Plant Genetics and Breeding Science Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan |
| Abstract: | The present study demonstrates utilization of 11 microsatellite markers to explore genetic diversity held in Perilla frutescens (L.) Britt. landrace accessions growing on farms in different parts of Korea and Japan and to assess their genetic relationships. All microsatellite loci were polymorphic and produced a total of 96 alleles ranging from 4 to 20, with an average of 8.7 alleles per locus. Of the 96 alleles found, a total of 15 unique landrace-specific alleles were observed at 9 different loci. The locus GBPFM203 provided the highest number of alleles (20), of which five were unique and each specific to a particular landrace accession. The occurrence of unique, accession-specific alleles presented molecular evidence for the generation of new alleles within on-farm collection of Perilla. The mean values of observed (H O) and expected heterozygosity (H E) were 0.39 and 0.68, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped the two Perilla varieties, var. frutescens and var. crispa (Thunb.) Decne into two distinct groups. Accessions belonging to var. frutescens could also be divided into two subgroups at a close genetic distance (GD = 0.432). The overall clustering pattern did not strictly follow the grouping of accessions according to their geographic origins. These observations are indicative of extensive germplasm exchange among farms from different geographical regions. The genetic similarity observed among the Perilla landraces may be useful for future Perilla crop variety identification, conservation, and improvement programs. |
| Keywords: | Genetic diversity Introgression Microsatellite markers Perilla frutescens var crispa var frutescens |
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