Immuno-electromicroscopic approach for the study of neural stem cell niches |
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| Authors: | Bonfanti L Aimar P Ponti G Canalia N |
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| Institution: | (1) Indian Veterinary Research Institute, Regional Station, Palampur, H.P., 176 061, India;(2) Division of Pathology, Indian Veterinary Research Institute, Izatnagar, U.P., 243 122, India |
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| Abstract: | Lymphoproliferative response (LPR) was studied in 19 lambs orally infected (Group I) with Mycobacterium avium subsp. paratuberculosis (MAP) with in vitro lymphocyte stimulation test using MTT dye reduction assay. The non-specific LPR against Con A and specific LPR against sonicated
antigen and johnin PPD (purified protein derivatives) were estimated on preinfection (0 day) and various days postinfection
period (15 to 330 dpi) in the animals, which were classified according to histological and bacteriological evidence of paratuberculosis
infection. Of the two antigens used, johnin PPD was found to be superior in terms of consistency and uniformity of response
over an observation period of about a year. Significantly (P < 0.05) higher LPR were observed in the infected sheep during
postinfection period, as compared with preinfection values and values from uninfected control sheep. It was evident from the
present study that the LPR in histologically infected animals fluctuated during the long course of infection and had a definite
relationship with the gut pathology and the mycobacterial load. The LPR were stronger but variable in sheep with grades 1,
2 and 3 lesions (paucibacillary) and increased progressively from 30 dpi onwards. The sheep with the advanced lesions (grade
4, multibacillary) showed progressive decline in LPR till 120 dpi after initial stronger response at 30 dpi. Most of the animals
were detected by LPR before initiation of faecal shedding of MAP. The results suggested that repeated testing was required
while screening an infected flock for detecting most of the positive animals. |
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| Keywords: | Cellular immune response Lymphocyte Stimulation Test Lymphoproliferative response Mycobacterium avium paratuberculosis Ovine Paratuberculosis Sheep |
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