| 45S rDNA-FISH鉴定银杏雌雄性别 |
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| 引用本文： | 张扬,朱胜男,金晓芬,洪义欢,陈建民.45S rDNA-FISH鉴定银杏雌雄性别[J].园艺学报,2007,34(6):1520-1524. |
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| 作者姓名： | 张扬 朱胜男 金晓芬 洪义欢 陈建民 |
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| 作者单位： | （扬州大学生物科学与技术学院，江苏扬州，225009） |
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| 基金项目： | 扬州大学校科研和校改项目;扬州大学学生科技创新计划项目 |
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| 摘 要： | 植物中核糖体基因主要位于染色体次缢痕的核仁组织区（NOR），NOR的数目与随体染色体数目相对应。以核糖体基因（45SrDNA）为探针，对明确的雌株和雄株银杏中期、间期细胞进行荧光原位杂交（FISH）。结果显示雌株有4个杂交点，雄株有3个杂交点，表明雌性银杏具有4条带随体的染色体，而雄性银杏具有3条带随体的染色体。进一步证明银杏的雌雄性别决定与随体染色体数目有关。
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| 关 键 词： | 银杏 核糖体基因 性别决定 荧光原位杂交 |
| 文章编号： | 0513-353X（2007）06-1520-05 |
| 收稿时间： | 2007-05-15 |
| 修稿时间： | 2007-08-27 |
Identification of Male and Female Ginkgo Plants by 45S rDNA-FISH |
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| ZHANG Yang,ZHU Sheng-nan,JIN Xiao-fen,HONG Yi-huan,CHEN Jian-min.Identification of Male and Female Ginkgo Plants by 45S rDNA-FISH[J].Acta Horticulturae Sinica,2007,34(6):1520-1524. |
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| Authors: | ZHANG Yang ZHU Sheng-nan JIN Xiao-fen HONG Yi-huan CHEN Jian-min |
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| Institution: | (College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, Jiangsu 225009, China) |
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| Abstract: | The ribosomal genes (45S rDNA) encoding ribosomal RNA synthesis were located in nuclear organizer region (NOR) in plants.The number of NOR should be corresponding to the number of chromosomes with satellite. The 45S rDNA was probed to the metaphase chromosomes and interphase nucleus from male and female ginkgo plants using fluorescent in situ hybridization (FISH). The metaphase chromosomes and interphase nucleus of female plants displayed four hybridization signals while male plants displayed three signals, indicating that the female plants had four chromosomes with satellite while the male plants had three chromosomes with satellite only. The sex determination of ginkgo plants was related likely with the number of chromosomes with satellite. |
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| Keywords: | Ginkgo biloba L Ribosomal gene Sex determination Fluorescent in situ hybridization |
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