Une technique ELISA pour la detection des anticorps anti-virus maedi-visna. Etude comparative avec l'immunodiffusion en gelose et la fixation du complementAn ELISA test for detection of maedi-visna antibodies. Comparative study with gel immunodiffusion and complement-fixation test期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Une technique ELISA pour la detection des anticorps anti-virus maedi-visna. Etude comparative avec l'immunodiffusion en gelose et la fixation du complementAn ELISA test for detection of maedi-visna antibodies. Comparative study with gel immunodiffusion and complement-fixation test

Authors:	C Vitu P Russo P Filippi R Vigne G Querat and A Giauffret

Institution:	^* Direction de la Qualité, Services Vétérinaires, Laboratoire National de Pathologie des Petits Ruminants, 63 Avenue des Arènes, 06051, Nice Cedex, France ^† Laboratoire de Virologie, Faculté de Médecine, Secteur Nord, 13326, Marseille Cedex 3, France

Abstract:	An indirect microELISA test was performed for detection of maedi-visna antibodies in ovine and caprine species. The antigen consisted in viral particles, highly purified by successive ultracentrifugations. By comparative testing of 934 sera in ELISA and gel immunodiffusion, we found a good correlation between these two te ts, and moreover, ELISA revealed another 11.3% of positive samples. The precocity of this ELISA was shown by experimental infection of sheep with different strains of maedi-visna: positive sera were detected 7 weeks post-infection, instead 4–5 months with gel immunodiffusion. The complement fixation test was compared with gel immunodiffusion and was found the less sensitive. This ELISA test appeared to be satisfactory, and may be used for early diagnosis of maedi-visna infection.

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