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| 黑曲霉β-葡萄糖苷酶水解大豆异黄酮糖苷研究 | |
| 引用本文： | 孙艳梅,张永忠,许晶,李文滨.黑曲霉β-葡萄糖苷酶水解大豆异黄酮糖苷研究[J].东北农业大学学报,2007,38(1):9-12. |
| 作者姓名： | 孙艳梅 张永忠 许晶 李文滨 |
| 作者单位： | 1. 东北农业大学理学院,黑龙江,哈尔滨,150030;国家教育部大豆生物学重点实验室,黑龙江,哈尔滨,150030 2. 东北农业大学理学院,黑龙江,哈尔滨,150030 3. 国家教育部大豆生物学重点实验室,黑龙江,哈尔滨,150030;东北农业大学大豆研究所,黑龙江,哈尔滨,150030 |
| 基金项目： | 国家科技专项基金，教育部重点实验室基金 |
| 摘 要： | 试验进行了黑曲霉生产β-葡萄糖苷酶研究;该黑曲霉β-葡萄糖苷酶有较高的热稳定性,50℃加热2h后酶活力减少为原来的92%。确立了该黑曲霉β-葡萄糖苷酶水解大豆异黄酮糖苷反应最佳方案;通过与酸水解和苦杏仁β-葡萄糖苷酶水解效率比较,发现该黑曲霉β-葡萄糖苷酶有较好的水解大豆异黄酮糖苷的能力。 |
| 关 键 词： | 黑曲霉 β-葡萄糖苷酶 大豆异黄酮 水解 |
| 文章编号： | 1005-9369（2007）01-0009-04 |
| 修稿时间： | 2005-07-01 |
Study on hydrolysis of soybean isoflavone glucosides by β-glucosidases from Aspergillus niger |
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| SUN Yanmei,ZHANG Yongzhong,XU Jing,LI Wenbin.Study on hydrolysis of soybean isoflavone glucosides by β-glucosidases from Aspergillus niger[J].Journal of Northeast Agricultural University,2007,38(1):9-12. | |
| Authors: | SUN Yanmei ZHANG Yongzhong XU Jing LI Wenbin |
| Institution: | 1. College of Science, Northeast Agricultural University, Harbin 150030, China; 2. Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, China; 3. Soybean Research hlstitute, Northeast Agricultural University, Harbin 150030, China |
| Abstract: | The production of β-glucosidases from Aspergillus niger was studied. The thermostability of β-glucosidase from Aspergillus niger was high. When the enzyme was incubated at 50 ℃ for 2 h, the remaining activity was 92% of the original level. The optimal conditions of β-glucosidase from Aspergillus niger to hydr-olyze isoflavones glycoside were established. Comparing with acid and β-glucosidase from almond, β-glucosidase from Aspergillus niger showed the highest hydrolysis efficiency. |
| Keywords: | Aspergillus niger β-glucosidases soybean isoflavone hydrolysis |
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