| 细叶石仙桃无菌播种与快速繁殖技术研究初报 |
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| 引用本文： | 刘保财,黄颖桢,赵云青,陈菁瑛.细叶石仙桃无菌播种与快速繁殖技术研究初报[J].福建农业学报,2014(5):461-464. |
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| 作者姓名： | 刘保财 黄颖桢 赵云青 陈菁瑛 |
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| 作者单位： | [1]福建省农业科学院药用植物研究中心,福建福州350003 [2]福建省农业科学院农业生物资源研究所,福建福州350003 |
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| 基金项目： | 福建省农业科学院导师制青年基金项目（2012DQB-28） |
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| 摘 要： | 为解决细叶石仙桃人工栽培种苗短缺问题，以细叶石仙桃蒴果为材料，应用植物组织培养技术，筛选不同阶段所需的最适培养基。结果表明其种子可在MS＋20 g·L^-1蔗糖＋8 g·L^-1琼脂培养基上萌发，发芽率可达70％以上；MS ＋50g·L^-1土豆＋20g·L^-1蔗糖＋8g·L^-1琼脂培养基有利于原球茎分化小苗，低浓度6-BA和NAA组合适合原球茎增殖与芽的分化；在1/2MS ＋0.5 mg·L^-1 NAA＋20 g·L^-1蔗糖＋8 g·L^-1琼脂培养基上生根率达100％，通过以上系列培养基的培养可获得完整植株。
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| 关 键 词： | 细叶石仙桃 种子 无菌播种 快速繁殖 |
A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe |
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| LIU Bao-cai,HUANG Ying-zhen,ZHAO Yun-qing,CHEN Jing-ying.A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe[J].Fujian Journal of Agricultural Sciences,2014(5):461-464. |
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| Authors: | LIU Bao-cai HUANG Ying-zhen ZHAO Yun-qing CHEN Jing-ying |
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| Institution: | 1. Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; 2. Institute of Agricultural t3ioresource, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China) |
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| Abstract: | To alleviate a seed shortage on Pholidota cantonensis Rolfe ,artificial culturing with medium optimization was investigated by using the plant tissue culture technology .The results showed that MS＋20 g · L^-1 sucrose＋8 g · L^-1 agar was an appropriate medium that allowed a germination rate above 70% .MS＋50 g · L^-1 potato＋20 g · L ^-1 sucrose＋8 g · L^ -1 agar was desirable for the plantlet′s protocorm differentiation .Low concentrations of 6-BA and NAA in medium was better for bud protocorm proliferation and differentiation .The medium formulated with 1/2 MS＋0.5 mg · L^-1 NAA＋20 g · L^-1 sucrose＋ 8 g · L ^-1 agar was optimal for rooting ,as a rate of 100%was achieved .By using the combination of the above-mentioned media at respective stages ,the plantlets of P . cantonensis Rolfe could be rapidly obtained from seeds . |
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| Keywords: | Pholidota cantonensis Rolfe seed aseptic seeding rapid propagation |
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