| 种番鸭源禽坦布苏病毒分离鉴定及基因组序列分析 |
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| 引用本文： | 傅光华,陈红梅,黄瑜,万春和,傅秋玲,程龙飞,施少华,林建生.种番鸭源禽坦布苏病毒分离鉴定及基因组序列分析[J].福建省农科院学报,2014(4):301-305. |
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| 作者姓名： | 傅光华 陈红梅 黄瑜 万春和 傅秋玲 程龙飞 施少华 林建生 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建福州350013 |
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| 基金项目： | 国家自然科学基金项目（31201936）;现代农业产业技术体系建设专项（CARS-43）;福建省种业创新与产业化工程项目（2011FJZY-9）;福建省科技计划项目-省属公益类科研院所基本科研专项（2011R1025-8） |
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| 摘 要： | 种番鸭是否感染禽坦布苏病毒颇受国内学者关注。本研究于国内外首次自表现产蛋下降的种番鸭卵巢中分离获得1株病毒（命名为WYZLJ-1359株），经RT-PCR方法鉴定为禽坦布苏病毒。经比较分析该病毒与我国其他禽源分离株全基因组序列表明，种番鸭源分离株WYZLJ-1359主要分子特征未出现变异，与2010年以来我国分离的其他禽源分离毒株的核苷酸序列同源性均在98.4％以上，且亲缘关系非常近，遗传距离均在1.8％以下，远低于与2000年以前分离的坦布苏病毒分离株之间的遗传距离。以上结果表明，我国禽坦布苏病毒感染的宿主在不断增加，但病毒在不同品种家禽的传播过程中遗传较为稳定，基因组未出现基因的缺失或插入等变异现象。
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| 关 键 词： | 坦布苏病毒 种番鸭 分离鉴定 基因组 |
Identification and Genome Sequence Analysis of Tembusu Virus Isolated From Breed Muscovy Duck |
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| Authors: | FU Guang-hua CHEN Hong-mei HUANG Yu WAN Chun-he FU Qiu-ling CHENG Long-fei SHI Shao-hua LIN Jian-sheng |
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| Institution: | ( Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China) |
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| Abstract: | Nowadays ,it is still unknow whether the avian tembusu virus could infect breed Muscovy duck .For the first time ,we obtained a viral isolate （named WYZLJ-1359） from ovary of breed Muscovy duck with decreasing egg-laying rate ,and confirmed the infectious agent as avian tembusu virus （ATV） using RT-PCR .Results of sequence comparison with viral genome showed that the main molecular characteristics of genome sequence of WYZLJ-1359 strain were in accordance with those of other viruses isolated from other domestic poultry since 2010 ,and shared no less than 98.4% nucleotide sequence identity with each other .Phylogenetic analysis showed that all ATVs isolated since 2010 shared closed relationship with each other .And the genetic distance （GD） among these viruses were all no more than 1.8% ,which was far less than the GD between WYZLJ-1359 strain and those viruses reported before 2000. These results indicated that although the host spectrum of avian tembusu virus continued to increase ,the virus showed genetic stability during the process of spread among different poultry species without deletion or insertion in viral genome . |
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| Keywords: | tembusu virus breed Muscovy duck isolation and identification genome |
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