| 金桔体细胞胚胎诱导的研究 |
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| 引用本文： | 黄靖,陈晓静.金桔体细胞胚胎诱导的研究[J].福建农业学报,2014(5):454-460. |
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| 作者姓名： | 黄靖 陈晓静 |
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| 作者单位： | [1]福建农业职业技术学院园艺园林系,福建福州350119 [2]福建农林大学园艺学院,福建福州350119 |
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| 摘 要： | 采用金桔无菌苗叶片为外植体，在单因素试验的基础上，通过正交试验研究不同生长调节剂对金桔胚性愈伤组织诱导的影响。结果表明：MS＋2，4-D2.0mg·L^-1＋BA0.5mg·L^-1处理最适宜金桔胚性愈伤组织诱导，其中淡黄色愈伤组织团块胚胎发生频率较高；在附加BA 0.05 mg·L^-1的MS培养基上淡黄色愈伤组织块可形成胚状体；胚状体试管苗在1/2MS＋NAA0.1 mg · L^-1培养基上，生根率可达80％，移栽成活率达70％
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| 关 键 词： | 金桔 组织培养 体细胞胚胎 |
Studying on Somatic Embryo Induction of Fortunella j aponica |
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| HUANG Jing,CHEN Xiao-jing.Studying on Somatic Embryo Induction of Fortunella j aponica[J].Fujian Journal of Agricultural Sciences,2014(5):454-460. |
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| Authors: | HUANG Jing CHEN Xiao-jing |
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| Institution: | 1. Fujian Agricultural Vocational and Technical College Department of Horticulture and Lanscape, Fuzhou, Fujian 350003, China; 2. College o f Horticulture , Fujian Agriculture and Forestry University, Fuzhou, Fujian 350003, China) |
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| Abstract: | Based on single factorial experiment ,the sterile seedlings of Fortunella japonica were used as explants for studying the effects of various growth regulators on inducing embryogeny with orthogonal test .The results showed：the MS ＋ 2 ,4-D 2.0 mg·L^-1 ＋BA 0.5 mg·L^-1 was the best culture medium for inducing embryonic callus .Sucrose at MS culture medium with BA 0.05 mg·L-1 gave a maximum embryogenesis from the light yellow callus tissue of leaf .Planting somatic embryos on 1/2MS culture medium with NAA0.1 mg·L^-1 ,the rooting rate was 80% ,the survive rate was 70% . |
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| Keywords: | Fortunella j aponica tissue culture somatic embryo |
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