| 一株引起香鱼出血症的嗜水气单胞菌的鉴定 |
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| 引用本文： | 张呈念,史雨红,李明云,陈炯.一株引起香鱼出血症的嗜水气单胞菌的鉴定[J].水产科学,2009,28(7). |
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| 作者姓名： | 张呈念 史雨红 李明云 陈炯 |
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| 作者单位： | 应用海洋生物技术教育部重点实验室,宁波大学,浙江,宁波,315211 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，教育部长江学者和创新团队发展计划 |
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| 摘 要： | 从发生出血症的香鱼体腔液分离到一高致病性的菌株(ayu-ah0201),经人工侵染试验证实该菌能引起香鱼出血症.对该菌的形态、生理生化及16S rDNA序列分析结果表明,其为革兰氏阴性,短杆状,极生鞭毛.对蔗糖、阿拉伯糖、七叶苷、氧化酶、精氨酸双水解酶、赖氨酸脱羧酶呈阳性,鸟氨酸脱羧酶呈阴性.可利用葡萄糖产酸产气.在以该菌16S rDNA序列和GenBank数据库内同源性较高的细菌16S rDNA序列构建的系统发育树,表明分离菌ayu-ah0201与嗜水气单胞菌聚在一簇,与嗜水气单胞菌核苷酸序列同源性最高,为99.6%～99.7%,与其他相关气单胞菌属成员的核苷酸序列同源性小于98.9%.结合形态和生理生化特点将其鉴定为嗜水气单胞菌.
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| 关 键 词： | 香鱼 出血症 系统发育 |
Isolation,Identification and Phylogenetic Analysis of a Bacterium Aeromonas hydrophila Causing Ayu(Plecoglossus altivelis)Hemorrhage Disease |
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| ZHANG Cheng-nian,SHI Yu-hong,LI Ming-yun,CHEN Jiong.Isolation,Identification and Phylogenetic Analysis of a Bacterium Aeromonas hydrophila Causing Ayu(Plecoglossus altivelis)Hemorrhage Disease[J].Fisheries Science,2009,28(7). |
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| Authors: | ZHANG Cheng-nian SHI Yu-hong LI Ming-yun CHEN Jiong |
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| Institution: | ZHANG Cheng-nian,SHI Yu-hong,LI Ming-yun,CHEN Jiong(Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China) |
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| Abstract: | A pathogenic bacterium(ayu-ah0201) was isolated from the coelomic fluid of ayu(Plecoglossus altivelis)with hemorrhage disease.Artificial infection proved that hemorrhage disease of the ayu was induced by this bacterium.The strain was examined for morphological,physiological,and biochemical characteristics,and 16S rDNA sequencing proved to be a kind of gram negative,with polar multi-flagella,ornithine decarboxylase-negative,glucose-positive,sucrose-positive,rhamnose-positive,esculin-positive,lysine decarboxy... |
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| Keywords: | 16S rDNA |
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