| 普通小麦-华山新麦草人工合成双二倍体AFLP标记多态性分析 |
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| 引用本文： | 袁璟亚,康厚扬,王益,钟美玉,周永红.普通小麦-华山新麦草人工合成双二倍体AFLP标记多态性分析[J].四川农业大学学报,2012(3):267-271. |
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| 作者姓名： | 袁璟亚 康厚扬 王益 钟美玉 周永红 |
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| 作者单位： | 四川农业大学小麦研究所 |
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| 基金项目： | 国家自然科学基金项目(31101151);国家科技计划(863)项目(2011AA100103) |
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| 摘 要： | 【目的】从分子水平上了解普通小麦-华山新麦草双二倍体(PHW-SA)合成过程中遗传变异情况。【方法】用195对引物对PHW-SA及其亲本普通小麦(J-11)和华山新麦草进行AFLP标记分析。【结果】J-11、华山新麦草和PHW-SA分别扩增出标记5 136、3 953和5 637条,分别占总标记(17 726条)的29.0%、22.3%和31.8%。81.8%的J-11标记和49.6%的华山新麦草标记存在于PHW-SA中。其中J-11与PHW-SA特有相同标记2 484条,分别占各自标记的48.4%和44.1%;华山新麦草与PHW-SA特有相同标记246条,分别占各自标记的6.2%和4.4%;PHW-SA特有标记232条,缺失双亲共有标记66条,分别占PHW-SA总标记的4.1%和1.2%;3种材料共有相同标记1 715条,分别占J-11、华山新麦草和PHW-SA总标记的33.4%、43.4%和30.4%。【结论】PHW-SA在合成过程中基因组发生了较大的遗传变异。此研究结果为PHW-SA在小麦遗传育种中的应用提供了基础理论依据。
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| 关 键 词： | 华山新麦草 AFLP标记 遗传变异 |
Polymorphism Analysis of Wheat-Psathyrostachys huashanica Amphiploids with AFLP |
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| YUAN Jing-ya,KANG Hou-yang,WANG Yi,ZHONG Mei-yu,ZHOU Yong-hong.Polymorphism Analysis of Wheat-Psathyrostachys huashanica Amphiploids with AFLP[J].Journal of Sichuan Agricultural University,2012(3):267-271. |
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| Authors: | YUAN Jing-ya KANG Hou-yang WANG Yi ZHONG Mei-yu ZHOU Yong-hong |
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| Institution: | *(Triticeae Research Institute,Sichuan Agricultural University,Wenjiang 611130,Sichuan,China) |
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| Abstract: | 【Objective】 The purpose of this study was to analyze the genetic variation of PHW-SA.【Method】 195 pairs of AFLP primers were used to amplify PHW-SA and its parents J-11(common wheat) and Psathyrostachys huashanica.【Results】 5 136,3 953 and 5 637 markers were amplified in J-11,Psathyrostachys huashanica and PHW-SA,respectively,accounted for 29.0%,22.3% and 31.8% of the total(17 726 banding),respectively;81.8% markers of J-11 and 49.6% markers of Psathyrostachys huashanica were amplified in PHW-SA;there were 2 484 co-markers between PHW-SA and J-11,which accounted for 48.4% and 44.1% of themselves,respectively;similarly,246 co-markers existed between(6.2%) PHW-SA and(4.4%) Psathyrostachys huashanica;232 new markers which did not exist in its parents were amplified in PHW-SA and 66 markers of its parents could not be amplified in PHW-SA;there were 1 715 co-markers in all 3 materials,accounted for 33.4%,43.4% and 30.4% of its total markers of J-11,Psathyrostachys huashanica and PHW-SA,respectively.【Conclusion】 All evidence suggest that great genetic variation was occurred in the genome of PHW-SA when synthesized,and provide the theoretical information for wheat breeding in future. |
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| Keywords: | Psathyrostachys huashanica AFLP marker hereditary variation |
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