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应用~(15)N标记技术研究栓皮栎氮素的吸收
引用本文:顾盛俊,周丕生,康宏樟.应用~(15)N标记技术研究栓皮栎氮素的吸收[J].上海交通大学学报(农业科学版),2016(3):69-73.
作者姓名:顾盛俊  周丕生  康宏樟
作者单位:上海交通大学 农业与生物学院,上海200240,上海交通大学 农业与生物学院,上海200240,上海交通大学 农业与生物学院,上海200240
基金项目:国家自然科学基金(31270491)
摘    要:应用~(15)N示踪技术,对盆栽栓皮栎进行外源氮素标记,并分析2年内栓皮栎叶片氮含量变化及外源氮素间的比例关系,揭示氮素去向,对木本植物氮利用进行深入研究。结果表明,栓皮栎叶片施用硝酸铵后4周内氮含量达到最高,之后生长期内氮含量会随着生长时间增加而显著下降,并可根据原产地分别建立回归方程;标记后第20周叶片~(15)N平均丰度达(7.89±1.35)%;栓皮栎对外源氮素有较高的利用率,施入肥料后第4周即趋于平衡,第16周达到峰值,平均肥料氮比例(Ndff%)为(7.77±0.69)%,在第56周的新叶中仍达(5.46±0.79)%,2个生长期同期的Ndff%无显著差异。不同产地栓皮栎的氮吸收利用规律较一致;原产安徽的栓皮栎在外源氮素利用率及氮吸收量两方面均表现良好。

关 键 词:15N标记    栓皮栎    氮素
收稿时间:2015/7/21 0:00:00

Study on Nitrogen Assimilation of Quercus variabilis with 15N Labeling
GU Sheng-jun,ZHOU Pi-sheng and KANG Hong-zhang.Study on Nitrogen Assimilation of Quercus variabilis with 15N Labeling[J].Journal of Shanghai Jiaotong University (Agricultural Science),2016(3):69-73.
Authors:GU Sheng-jun  ZHOU Pi-sheng and KANG Hong-zhang
Institution:School of Agricultureand Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agricultureand Biology,Shanghai Jiaotong University,Shanghai 200240,China and School of Agricultureand Biology,Shanghai Jiaotong University,Shanghai 200240,China
Abstract:The 15N trace technology was employed to study the nitrogen assimilation in Quercus variabilis leaves within 2 years.The change of nitrogen content and exogenous nitrogen proportion in leaves was analyzed to reveal the transference of nitrogen in woody plant.The results showed that the nitrogen content of Quercus variabilis leaves reached the highest level in the 4th week after being labeled,and then decreased significantly as time passed in the whole year,which could be portrayed by regression equations based on the origin,respectively.The average 15N abundance in leaves was (7.89±1.35)% in the 20th week after being labeled.Quercus variabilis has efficient utilization of exogenous nitrogen that the assimilation had been in balance since the 4th week after being fertilized and reached peak in the 16th week.The average percentage of nitrogen from the fertilizer(Ndff%)was (7.77±0.69)% in the 16th week,and still remained (5.46±0.79)% in the 56th week.The Ndff% in the second year remained as same as the corresponding period in the first year.Quercus variabilis from different origins showed similar rule in nitrogen assimilation.The species from Anhui performed well in both exogenous nitrogen utilization and nitrogen content increase.
Keywords:15N  Quercus variabilis Bl    nitrogen
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