Effect of sinapine on H2O2-induced adipogenic differentiation of bone marrow mesenchymal stem cells |
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| Authors: | HUANG Wei-ping LI Ke LIU Ai-jun CHEN Dong-feng WANG Hong-qi |
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| Institution: | College of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | AIM:To investigate the effects of sinapine, an effective monomer of Chinese medicine, on hydrogen peroxide (H2O2)-induced adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).METHODS:The undifferentiated rat BMSCs were identified and screened by flow cytometry. The adipogenic differentiation of BMSCs was induced by H2O2, and the toxicity of sinapine on BMSCs was tested by CCK-8 assay. After the modeling method and the concentration range of sinapine were determined, the lipid droplets in the cells were detected by Oil Red O semi-quantitative assay, and the optimal drug concentration was selected. Finally, Oil Red O assay was observed 24 h after drug intervention, and the expression of adipogenic differentiation-related proteins, adipocyte protein 2 (aP2), peroxisome proliferator-activated receptor γ (PPARγ) and glucose transporter 4 (Glut4), at mRNA and protein levels in the BMSCs was determined by qPCR and Western blot.RESULTS:Treatment with H2O2 at 200 μmol/L for 1 h induced BMSCs to differentiate into adipocytes. Below the concentration of 40 μmol/L, sinapine had no toxicity to BMSCs. The best inhibitory concentration of sinapine on adipogenic differentiation was at 15 μmol/L. The number of lipid droplets in sinapine (15 μmol/L) group was significantly lower than that in model group. In sinapine group, the expression of aP2, PPARγ and Glut4 at mRNA and protein levels was lower than that in model group (P<0.01).CONCLUSION:Sinapine inhibits H2O2-induced adipogenic differentiation of rat BMSCs. The mechanism may be related to the PPARγ/AMPK signaling pathway. |
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| Keywords: | Sinapine Bone marrow mesenchymal stem cells Adipogenic differentiation Hydrogen peroxide Oxidative stress |
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