IL-17A reduces lipid accumulation by up-regulation of ABCA1 expression in Raw264.7 macrophages |
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| Authors: | MA Wang-ge ZHOU Dong WANG Li-jun LIU Yan CHEN Xiao-li GUO Ning ZHOU Juan |
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| Institution: | 1. Department of Cardiovascular Medicine, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710061, China;
2. Department of Cardiovascular Medicine, Hanzhong Central Hospital, Hanzhong 723000, China |
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| Abstract: | AIM:To investigate the effects of interleukin-17A (IL-17A) on the expression of adenosine triphosphate binding cassette transporter A1 (ABCA1) in RAW264.7 macrophages. METHODS:Mouse RAW264.7 macrophages were treated with IL-17A at different concentrations for 6 or 24 h, or treated with IL-17A at the same concentration for different time. The expression of ABCA1 at mRNA and protein levels was determined by RT-qPCR and Western blot, respectively. Cholesterol efflux to apolipoprotein A1 (ApoA-1) was evaluated by NBD-cholesterol method. Lipid accumulation in the cells was evaluated by Oil Red O staining. RESULTS:Compared with control group, IL-17A increased the expression of ABCA1 at protein level in the RAW264.7 cells significantly (P<0.05), but had no effect on the mRNA expression of ABCA1. In addition, cholesterol efflux to ApoA-1 was increased and lipid accumulation in the RAW264.7 cells was decreased obviously after treatment with IL-17A. CONCLUSION:IL-17A increases the protein expression of ABCA1 but not at mRNA level in the RAW264.7 macrophages, which may be correlated with its anti-atherosclerosis effect. |
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| Keywords: | Interleukin-17A RAW264 7 macrophages Adenosine triphosphate binding cassette transporter A1 Lipid accumulation |
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