| 枯草芽孢杆菌NBF809防治番茄棒孢叶斑病研究 |
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| 引用本文： | 黄大野,曹春霞,张亚妮,王开梅,饶,犇,万中义,周荣华,杨自文.枯草芽孢杆菌NBF809防治番茄棒孢叶斑病研究[J].中国蔬菜,2018,1(12):40-44. |
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| 作者姓名： | 黄大野 曹春霞 张亚妮 王开梅 饶 犇 万中义 周荣华 杨自文 |
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| 作者单位： | （湖北省生物农药工程研究中心，湖北武汉430064） |
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| 基金项目： | 国家重点研发计划项目（2017YFD0201107），湖北省自然科学基金重点类项目（2016ABA103），湖北省农业科技创新中心创新团队项目（2016-620-000-001-038） |
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| 摘 要： | 采用平板对峙结合显微观察确定枯草芽孢杆菌NBF809菌株对多主棒孢的抑菌活性,采用活体盆栽试验研究其对番茄棒孢叶斑病的防治效果。结果表明,在平板对峙条件下NBF809菌株对多主棒孢具有良好的抑菌活性,显微镜下观察到菌丝发生扭曲、肿胀和变形;盆栽试验结果表明NBF809菌株对番茄棒孢叶斑病的防治效果达到73.26%。聚合酶链式反应用于检测NBF809菌株的抗菌素生物合成基因,扩增到bac D、itu C、itu D、mrs M和myc C 5个基因,涉及Bacilysin、Iturin、Mersacidin和Mycosubtilin 4种抗菌素的合成。
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| 关 键 词： | 枯草芽孢杆菌 番茄 棒孢叶斑病 抗菌素 |
Studies on Controlling Tomato Corynespora Leaf Spot by Bacillus subtilis NBF809 |
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| HUANG Da-ye,CAO Chun-xia,ZHANG Ya-ni,WANG Kai-mei,RAO Ben,WAN Zhong-yi,ZHOU Rong-hua,YANG Zi-wen.Studies on Controlling Tomato Corynespora Leaf Spot by Bacillus subtilis NBF809[J].China Vegetables,2018,1(12):40-44. |
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| Authors: | HUANG Da-ye CAO Chun-xia ZHANG Ya-ni WANG Kai-mei RAO Ben WAN Zhong-yi ZHOU Rong-hua YANG Zi-wen |
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| Institution: | （Biopesticide Engineering Research Center of Hubei Province，Wuhan 430064，Hubei，China） |
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| Abstract: | The inhibition activity was tested by dual culture method and microscope observation，and the
control efficacy of Bacillus subtilis NBF809 on tomato Corynespora leaf spot was investigated by pot experiment.
The results showed that NBF809 strain had good inhibition activity in dual culture. The distortion，swelling and
deformation of mycelium were observed under microscope. Result of pot experiment demonstrated that the control
efficacy of NBF809 strain on tomato Corynespora leaf spot reached 73.26%. Polymerase chain reaction was used
to detect antibiotics produced by NBF809 strain. These antibiotics were amplified to bacD，ituC，ituD，mrsM
and mycC 5 genes，involving respectively in the biosynthesis of 4 antibiotics：Bacilysin，Iturin，Mersacidin and
Mycosubtilin. |
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| Keywords: | Bacillus subtilis Tomato Corynespora leaf spot Antibiotics |
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