| 常用中草药对水产动物致病菌体外抑菌效果研究 |
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| 引用本文： | 王洪彬,朱利霞,杨楠,吴同垒,张志强,高桂生,史秋梅.常用中草药对水产动物致病菌体外抑菌效果研究[J].中国畜牧兽医,2018,45(5):1357-1366. |
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| 作者姓名： | 王洪彬 朱利霞 杨楠 吴同垒 张志强 高桂生 史秋梅 |
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| 作者单位： | 河北科技师范学院动物科技学院, 河北省预防兽医学重点实验室, 秦皇岛 066604 |
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| 基金项目： | 秦皇岛市科技局项目（201602A341） |
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| 摘 要： | 试验旨在探讨黄柏、诃子、知母、苏木等常用中草药对水产动物致病菌的体外抑菌效果，为今后水产养殖细菌性疾病的防控提供参考。以美人鱼发光杆菌、嗜水气单胞菌、哈维氏弧菌、创伤弧菌临床分离株为试验菌株，以抑菌圈直径、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）及联合抑菌指数（FICI）作为检测指标，研究常用中草药对水产病原菌的抑菌活性。采用水提法提取中草药有效成分，并通过琼脂平板打孔法测定中草药对水产致病菌抑菌圈，微量肉汤稀释法测定中草药MIC和MBC及微量肉汤棋盘稀释法测定联合抑菌指数（FICI）。结果显示，16种中草药对水产致病菌具有不同程度的抑菌作用。药敏试验结果显示，苏木、女贞子、夏枯草、青皮对4种水产致病菌均具有抑菌效果，抑菌圈直径在10.5~29.0 mm之间，而4种病原菌对马齿苋均表现为耐药；MIC检测结果显示，苏木、女贞子、夏枯草、青皮对4种病原菌的MIC值为7.81~250.00 mg/mL；MBC检测结果显示，苏木、女贞子、夏枯草、青皮对4种病原菌的MBC值为7.81~500.00 mg/mL；联合抑菌试验结果显示，苏木与夏枯草、女贞子及青皮联用仅对美人鱼发光杆菌起到协同抑菌作用，对嗜水气单胞菌、创伤弧菌及哈维氏弧菌均无抑菌作用，女贞子和青皮组合仅对哈维氏弧菌抑菌作用为相加作用，该组合在抑菌效果上对其他病原菌表现为无关或颉颃作用，其他药物组合在抑菌效果上对4种病原菌则表现为无关或颉颃作用；联合抑菌圈测定结果表明，中草药联合使用对4种病原菌抑菌作用不一。本试验结果表明常用中草药对水产动物致病菌具有一定程度的抑菌作用。
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| 关 键 词： | 中草药 体外抑菌效果 微量肉汤稀释法 最小抑菌浓度(MIC) 最小杀菌浓度(MBC) |
| 收稿时间： | 2017-10-24 |
Study on Antibacterial Effects of Common Chinese Herbs Against Aquatic Animal Pathogens in vitro |
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| WANG Hongbin,ZHU Lixia,YANG Nan,WU Tonglei,ZHANG Zhiqiang,GAO Guisheng,SHI Qiumei.Study on Antibacterial Effects of Common Chinese Herbs Against Aquatic Animal Pathogens in vitro[J].China Animal Husbandry & Veterinary Medicine,2018,45(5):1357-1366. |
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| Authors: | WANG Hongbin ZHU Lixia YANG Nan WU Tonglei ZHANG Zhiqiang GAO Guisheng SHI Qiumei |
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| Institution: | Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China |
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| Abstract: | The experiment was aimed to study antibacterial effect of common Chinese herbs such as Phellodendron amurense,Terminalia chebula,Rhizoma anemarrhenae,Caesalpinia sappan etc.on aquatic animal pathogenic bacteria in vitro and provide reference for the prevention and control of bacterial diseases in aquaculture.In this experiment,Photobacterium damsela,Aeromonas hydrophila,Vibrio harveyi and Vibrio vulnificus isolates were the test strains,the diameter of inhibition zone,minimum inhibitory concentration (MIC),minimum bactericidal concentration (MBC) and fractional inhibitory concentration index (FICI) were detected to study the antibacterial activity of Chinese herbal medicine on common aquatic pathogenic bacteria.Effective ingredients of Chinese herbal medicine was extracted by water,the inhibitory effect of Chinese herbal medicine on aquatic pathogenic bacteria was determined by agar plate punching method,the MIC and MBC of Chinese herbal medicine were determined by microbroth dilution method,the FICI was determined by broth microdilution method.The results showed that 16 kinds of Chinese herbal medicine had different degrees of bacteriostasis to aquatic pathogenic bacteria.Drug sensitivity test showed that Caesalpinia sappan,Ligustrum lucidum,Prunella vulgaris and Pericarpium citri reticulatae viride had bacteriostatic effect on 4 kinds of aquatic pathogenic bacteria,the inhibition zone diameter were from 10.5 to 29.0 mm,and all 4 pathogenic bacteria were resistant to Portulaca oleracea;The MIC test results showed that the MIC of Caesalpinia sappan,Ligustrum lucidum,Prunella vulgaris and Pericarpium citri reticulatae viride on 4 kinds of pathogenic bacteria were from 7.81 to 250.00 mg/mL;The MBC of Caesalpinia sappan,Ligustrum lucidum,Prunella vulgaris and Pericarpium citri reticulatae viride on 4 kinds of pathogenic bacteria were from 7.81 to 500.00 mg/mL.Combined antimicrobial test results showed that with Caesalpinia sappan,Prunella vulgaris,Ligustrum lucidum and Pericarpium citri reticulatae viride combined with only Photobacterium damsela played a synergistic inhibitory effect,no inhibitory effect on Aeromonas hydrophila,Vibrio vulnificus and Vibrio harveyi.The combination of Ligustrum lucidum and Pericarpium citri reticulatae viride had an additive effect on the bacteriostatic action of Vibrio harveyi only,the combination of other pathogenic bacteria in the inhibitory effect showed independent or antagonistic effects,other drug combinations in the 4 kinds of pathogenic bacteria antibacterial effect on performance of unrelated effect or antagonistic effect.The results of the combined inhibition zone showed that the combination of Chinese herbal medicine had different bacteriostatic effects on the four pathogens.The results showed that common Chinese herbal medicine had a certain inhibitory effect on the aquatic pathogens. |
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| Keywords: | Chinese herbal medicine in vitro antibacterial effect microbroth dilution method minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) |
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