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| 复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响 | |
| 引用本文： | 徐海旺,余魁,吴梦郡,刘文凯,李鹏,易丹,王蕾,侯永清.复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响[J].中国畜牧兽医,2018,45(8):2197-2203. |
| 作者姓名： | 徐海旺 余魁 吴梦郡 刘文凯 李鹏 易丹 王蕾 侯永清 |
| 作者单位： | 1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023; 2. 武汉轻工大学动物营养与饲料安全湖北省协同创新中心, 武汉 430023 |
| 基金项目： | 武汉市科技计划项目（2016020101010080）；国家重点研发计划专项（2016YFD0501210）；湖北省技术创新专项（重大项目）（2016ABA121） |
| 摘 要： | 试验旨在研究复合植物精油（OCT）对脂多糖（LPS）诱导的断奶仔猪的免疫应激的影响。试验选取28日龄左右的杜×长×大三元杂交仔猪，随机分成3个处理组：对照组（基础日粮，注射生理盐水），LPS组（基础日粮，注射LPS），OCT组（基础日粮+50 mg/kg OCT，注射LPS）。每组8个重复，每个重复1头猪。于试验第21天注射LPS（100 μg/kg体重）或等体积的生理盐水，注射3 h后前腔静脉采血进行白细胞分类计数；6 h后屠宰，取胸腺、脾脏，-80℃保存，用于测量胸腺、脾脏免疫相关基因白介素-4（IL-4）、白介素-6（IL-6）、白介素-10（IL-10）、肿瘤坏死因子（TNF-α）、核因子（NF-κB）以及转录相关基因转录激活因子3（STAT3）的基因相对表达量。结果表明：①与对照组相比，LPS刺激降低了仔猪血液中白细胞、中性粒细胞、淋巴细胞、单核细胞及嗜酸性粒细胞数量（P<0.05）；与LPS组相比，日粮中添加OCT提高了白细胞的数量（P<0.05）；②与对照组相比，LPS刺激上调了脾脏、胸腺IL-6、STAT3 mRNA水平（P<0.05），下调了脾脏IL-4、TNF-α及胸腺IL-4、TNF-α、NF-κB mRNA水平（P<0.05）；与LPS组相比，添加OCT下调了脾脏、胸腺IL-6、STAT3 mRNA水平（P<0.05）；上调了胸腺IL-4、IL-10、TNF-α、NF-κB的mRNA水平（P<0.05）。综合上述结果，LPS诱导发生了仔猪免疫应激，OCT在一定程度具有缓解作用。 |
| 关 键 词： | 复合植物精油(OCT) 断奶仔猪 脂多糖(LPS) 免疫应激 |
| 收稿时间： | 2017-11-26 |
The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs |
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| XU Haiwang,YU Kui,WU Mengjun,LIU Wenkai,LI Peng,YI Dan,WANG Lei,HOU Yongqing.The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs[J].China Animal Husbandry & Veterinary Medicine,2018,45(8):2197-2203. | |
| Authors: | XU Haiwang YU Kui WU Mengjun LIU Wenkai LI Peng YI Dan WANG Lei HOU Yongqing |
| Institution: | 1. Hubei key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China; 2. Hubei Collaborative Innovation Center of Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China |
| Abstract: | The purpose of this research was to study the effect of OCT on the immune stress of weaned piglets induced by LPS.A total of 24 weaned piglets (Duroc×Large white×Landrace) were randomly assigned to three treatments at 28 days:Control group (based diet,injection of saline),LPS group (basic diet,injection of LPS) and OCT group (basal diet+50 mg/kg OCT,injection of LPS).Each treatment included 8 piglets that were subdivided equally to 8 pens,each pen had 1 piglets.On 21th day of the experiment,piglets in the LPS and OCT groups were injected intraperitoneally with LPS (100 μg/(kg·BW)),whereas piglets in the control group were injected intraperitoneally with the same volume of physiological saline.Blood samples were obtained 3 h after injection for analysis of white blood cell differential counts.6 h after LPS challenged,the pigs were slaughtered and thymus and spleen were taken for measuring the related genes IL-4,IL-6,IL-10,TNF-α,NF-κB,and STAT3 mRNA.The result showed that:①Compared with control group,LPS stimulation could lead to a significant reduction in the number of the eosinophils,neutrophils,lymphocytes,monocytes,white blood cells in the blood (P<0.05).Compared with LPS group,adding OCT could significantly increase blood levels of white blood cells (P<0.05).②Compared with control group,after injection of LPS,mRNA levels of IL-6 and STAT3 genes significantly increased in spleen and thymus (P<0.05);mRNA levels of IL-4,TNF-α in spleen and IL-4,TNF-α,NF-κB genes mRNA levels in thymus had been significantly reducted (P<0.05).Compared with LPS group,adding OCT could significantly reduce IL-6 and STAT3 mRNA levels in both spleen and thymus (P<0.05),and significantly increase IL-4,IL-10,TNF-α and NF-κB genes mRNA levels in thymus (P<0.05).In conclusion,LPS could induce immune stress in weaned pigs,whereas OCT could improve its immunity and alleviate inflammation to some extent. |
| Keywords: | compound plant of essential oil (OCT) piglets lipopolysaccharide(LPS) immune stressed |
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