Baicalin inhibits high glucose-induced apoptosis of mouse glomerular mesangial cells via miR-141/Sirt1 signaling pathway |
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| Authors: | WU Jun XIA Yuan-yu CHEN Jie XIAO Ling WANG You ZHAO Yuan-yuan YE Gang YIN Qing-qiao |
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| Institution: | 1. Department of Endocrinology, The Third Hospital of Wuhan, Wuhan 430000, China;
2. Department of Nephrology, The Third Hospital of Wuhan, Wuhan 430000, China |
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| Abstract: | AIM:To investigate the therapeutic mechanism of baicalin for diabetic nephropathy involving microRNA-141 (miR-141)/silent information regulator 1 (Sirt1) signaling pathway. METHODS:Mouse glomerular mesan-gial cell line SV40-MES-13 was treated with high glucose (HG, 25 mmol/L glucose) to establish diabetic nephropathy cell model. Baicalin at 100 μmol/L was used to treat glomerular mesangial cells. qPCR and Western blot were performed to determine the expression levels of miR-141 and Sirt1. The regulatory relationship between miR-141 and Sirt1 was detected by dual-luciferase assay. The apoptosis of glomerular mesangial cells was analyzed by flow cytometry. RESULTS:Compared with control group, the cells treated with HG showed increased levels of miR-141 and apoptosis, and Sirt1 expression was decreased (P<0.01). Baicalin and miR-141 inhibitor suppressed the HG-induced effect on the levels of miR-141, Sirt1 and apoptosis. Knockdown of Sirt1 expression reversed the effect of miR-141 inhibitor on the levels of miR-141, Sirt1 and apoptosis. Over-expression of miR-141 reversed the effect of baicalin on the glomerular mesangial cells treated with HG. Up-regulation of Sirt1 abolished the effect of miR-141 over-expression on the glomerular mesangial cells. CONCLUSION:Baicalin inhibits the apoptosis of mouse glomerular mesangial cells via miR-141/Sirt1 signaling pathway, thus attenuating diabetic nephropathy. |
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| Keywords: | Diabetic nephropathy Apoptosis Baicalin MicroRNA-141 Silent information regulator 1 |
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