Effects of High Alternating Day and Night Temperature Conditions on the Boll Wall's Bt Protein Content and Nitrogen Metabolic Physiology in Bt Cotton |
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| Institution: | Key Laboratory of Genetics and Physiology in Yangzhou University, Yangzhou, Jiangsu 225009, China |
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| Abstract: | Object] In this study, the objective was to study changes in the boll wall's Bt protein concentration and related physiological characteristics under high alternating day and night temperature conditions in Bt cotton. Method] The experiments were conducted at the Key Laboratory of Genetics and Physiology, Yangzhou University during the 2011-2012 cotton growing seasons. The conventional Bt cotton cultivar Sikang-1 and hybrid cultivar Sikang-3 were used as the test materials. The boll wall's Bt protein content and nitrogen metabolic physiology were investigated under high temperatures (39/27 ℃, day/night) for 4-10 d at the peak boll stage. Result] The Bt protein content in the boll wall decreased as the stress time increased. Compared with the control (day/night, 32/25 ℃), the Bt protein content in the boll wall decreased significantly at 7 d under the high temperature stress. The values for cultivars Sikang-1 and Sikang-3 decreased by 18.44% and 14.82%, respectively, in 2011, and by 19.07% and 15.26%, respectively, in 2012. As the Bt toxin content decreased, the boll wall's free amino acid content and protease activity increased, while the soluble protein content and GPT (Glutamic-pyruvic transaminase) activity decreased significantly. Conclusion] The reduction in Bt protein synthesis and increase in the insecticidal protein degradation at the boll wall under high temperatures caused a reduction in the protein content, including the Bt protein content, which resulted in a reduction in insect resistance. |
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| Keywords: | Bt cotton high temperature stress Bt protein nitrogen metabolism boll wall |
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