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| 摩拉水牛和尼里-拉菲水牛PRKAA2基因SNPs检测及遗传多样性分析 | |
| 引用本文： | 黄玥萌,郑海英,杨春艳,黄加祥,鄢胜飞,李舒露,于农淇,李孟琪,尚江华.摩拉水牛和尼里-拉菲水牛PRKAA2基因SNPs检测及遗传多样性分析[J].中国畜牧兽医,2018,45(5):1274-1282. |
| 作者姓名： | 黄玥萌 郑海英 杨春艳 黄加祥 鄢胜飞 李舒露 于农淇 李孟琪 尚江华 |
| 作者单位： | 1. 农业部(广西)水牛遗传繁育重点实验室, 南宁 530001; 2. 中国农业科学院广西水牛研究所, 南宁 530001; 3. 西北农林科技大学动物医学院, 杨凌 712100 |
| 基金项目： | 广西科技计划项目（桂科AB16380040）；广西水产畜牧兽医局科技项目（桂渔牧科（201452029、201528016、201633006））；广西水牛研究所基本科研业务费（水牛基160204）） |
| 摘 要： | 为研究水牛蛋白激酶AMP活化的催化亚基α2（protein kinase AMP-activated catalytic subunit alpha 2，PRKAA2）基因多态性，本试验以摩拉水牛和尼里-拉菲水牛基因组DNA为模板，扩增PRKAA2基因外显子4及内含子3部分序列，通过常规测序法检测其SNP并进行遗传多样性分析。结果发现，PRKAA2基因外显子4内存在1个SNP位点（c.462 G>A），PRKAA2基因内含子3部分序列存在3个SNPs位点（IVS3.557 T>C、IVS3.560 C>T和IVS3.565 G>A）。经遗传多样性分析表明，在c.462 G>A位点的野生纯合型和杂合型比突变纯合型更有优势，IVS3.557 T>C和IVS3.560 C>T位点的突变纯合型为非优势基因型，IVS3.565 G>A位点杂合型为优势基因型。IVS3.565 G>A位点在摩拉水牛群体中处于Hardy-Weinberg非平衡状态；c.462 G>A位点在尼里-拉菲水牛群体中处于Hardy-Weinberg非平衡状态。4个SNPs位点在摩拉水牛群体中均为中度多态；c.462 G>A、IVS3.557 T>C位点在尼里-拉菲水牛群体中为低度多态，IVS3.560 C>T、IVS3.565 G>A位点为中度多态。IVS3.557 T>C位点在两个水牛群体中杂合度较低。说明摩拉水牛IVS3.565 G>A位点和尼里-拉菲水牛c.462 G>A位点的基因型频率和基因频率遗传状态不平衡，尼里-拉菲水牛群体中IVS3.557 T>C位点遗传变异小，选择潜力不高。4个多态位点可以构建5种单倍型，其中T-C-G-G是摩拉水牛群体和尼里-拉菲水牛群体的优势单倍型。综上，本研究检测的摩拉水牛和尼里-拉菲水牛PRKAA2基因上4个SNPs位点可为水牛标记辅助选择育种提供参考。 |
| 关 键 词： | 摩拉水牛 尼里-拉菲水牛 PRKAA2基因 SNP 常规测序 |
| 收稿时间： | 2017-10-16 |
SNPs Detection and Genetic Diversity Analysis of PRKAA2 Gene in Murrah and Nili-Ravi Buffaloes |
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| HUANG Yuemeng,ZHENG Haiying,YANG Chunyan,HUANG Jiaxiang,YAN Shengfei,LI Shulu,YU Nongqi,LI Mengqi,SHANG Jianghua.SNPs Detection and Genetic Diversity Analysis of PRKAA2 Gene in Murrah and Nili-Ravi Buffaloes[J].China Animal Husbandry & Veterinary Medicine,2018,45(5):1274-1282. | |
| Authors: | HUANG Yuemeng ZHENG Haiying YANG Chunyan HUANG Jiaxiang YAN Shengfei LI Shulu YU Nongqi LI Mengqi SHANG Jianghua |
| Institution: | 1. Guangxi Key Laboratory of Buffalo Genetics, Breeding and Reproduction, Nanning 530001, China; 2. Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Science, Nanning 530001, China; 3. College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China |
| Abstract: | In order to study the polymorphism of protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2),the genome DNA of Murrah and Nili-Ravi buffaloes were used as templates to amplify exon 4 and intron 3 partial sequences of PRKAA2 gene,the SNPs were detected by routine sequencing,and the genetic diversity was analyzed.The results showed that there was 1 SNP (c.462 G>A) in exon 4 and 3 SNPs (IVS3.557 T>C,IVS3.560 C>T and IVS3.565 G>A) in the intron 3.The genetic diversity results showed that wild homozygote and heterozygote were more advantages than mutant homozygote at c.462 G>A,the mutation homozygous genotypes were non-dominant genotypes at IVS3.557 T>C and IVS3.560 C>T,and the heterozygous genotypes were dominant genotypes at IVS3.565 G>A.IVS3.565 G>A in Murrah buffaloes and c.462 G>A in Nili-Ravi buffaloes were in non-equilibrium.All of 4 SNPs in Murrah buffaloes were at moderate polymorphism,c.462 G>A and IVS3.557 T>C in Nili-Ravi buffaloes were at low polymorphism,and IVS3.560 C>T and IVS3.565 G>A were at moderate polymorphism.The heterozygosity of IVS3.557 T>C was lower in two buffalo populations.The genotype frequency and gene frequency of IVS3.565 G>A in Murrah buffalo and c.462 G>A in Nile-Rafi buffalo were imbalanced,the genetic variation of IVS3.557 T>C in Nile-Rafi buffalo was low,without a high selection potential.4 SNPs would be combined into 5 haplotypes,T-C-G-G type was the dominant haplotype in two buffalo populations.In conclusion,4 SNPs of PRKAA2 gene in Murrah and Nili-Rafi buffaloes could provide a reference for marker-assisted selection breeding. |
| Keywords: | Murrah buffalo Nili-Ravi buffalo PRKAA2 gene SNP routine sequencing |
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