Generation and Glyphosate Resistance Analysis of G10eve Transgenic Cotton |
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| Authors: | Lu Guoqing Wang Chunling Hao Yuqiong Guo Huiming Huang Yingjin Cheng Hongmei |
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| Institution: | 1. College of Agronomy, Jiangxi Agricultural University/ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, China; 2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Objective] Novel germplasm of cotton was generated for development of cultivars with high resistance to glyphosate. Method] The glyphosate resistance gene G10eve was preliminarily analyzed using bioinformatics methods and introduced into 'Coker 312' via Agrobacterium-mediated transformation. The expression of G10eve was estimated by PCR (Polymerase chain reaction) and qRT-PCR (Quantitative real-time PCR). The content of shikimic acid and tolerance to glyphosate were determined in response to application of glyphosate to 'Coker 312' and transgenic plants. Result] A total of 28 independent positive transgenic lines were obtained. The transformation efficiency and the rate of seedling differentiation were up to 49.3% and 40.6%, respectively. PCR and qRT-PCR analysis showed that G10eve was integrated into the cotton genome, and the G10eve expression level differed significantly among the transgenic lines. At the cotyledon stage, the transgenic cotton showed resistance to 8 mL·L－1 glyphosate, whereas 'Coker 312' showed phytotoxicity to the 2 mL·L－1 glyphosate treatment. No significant accumulation of shikimic acid was detected in transgenic plants before or after glyphosate treatment, which indicated the typical physical characteristic of glyphosate resistance. Conclusion] Overexpression of G10eve in cotton improved resistance to glyphosate. Thus, novel germplasm of cotton resistant to glyphosate was generated. |
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| Keywords: | cotton G10eve glyphosate resistance |
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