|
|||||
|
|
| 不同生物素水平对3T3-L1脂肪细胞脂肪分解及相关基因表达的影响 | |
| 引用本文： | 雒诚龙,邵伟,任万平,赵艳坤,武开乐,余雄.不同生物素水平对3T3-L1脂肪细胞脂肪分解及相关基因表达的影响[J].中国畜牧兽医,2018,45(7):1783-1790. |
| 作者姓名： | 雒诚龙 邵伟 任万平 赵艳坤 武开乐 余雄 |
| 作者单位： | 1. 新疆农业大学动物科学学院, 乌鲁木齐 830052; 2. 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052 |
| 基金项目： | 国家自然科学基金（31560645）；现代农业产业技术体系建设专项资金资助（CARS-37）；新疆维吾尔自治区重点实验室开放课题（2015KL018）；中国博士后基金委资助 |
| 摘 要： | 为研究不同生物素水平对3T3-L1脂肪细胞脂肪分解及相关基因表达的影响，本试验利用三联诱导法将3T3-L1细胞经诱导分化为3T3-L1脂肪细胞。当3T3-L1脂肪细胞密集后分别采用0（对照组）、0.2、0.5、1.0 μmol/L生物素处理，分别在12、24与48 h时检测细胞上清液中甘油释放量、脂肪甘油三酯水解酶（ATGL）、激素敏感性脂肪酶（HSL）、脂滴包被蛋白A （perilipin A）及脂肪分化相关蛋白（ADRP）相对表达量。结果显示，在12 h时，1.0 μmol/L组甘油释放量、ATGL基因mRNA相对表达量均极显著低于对照组（P<0.01），0.5、1.0 μmol/L组HSL基因mRNA相对表达量显著低于对照组（P<0.05），1.0 μmol/L组perilipin A、ADRP基因mRNA相对表达量均极显著高于对照组与0.2 μmol/L组（P<0.01）；在24 h时，各试验组甘油释放量、1.0 μmol/L组ATGL基因mRNA相对表达量均极显著低于对照组（P<0.01），0.5、1.0 μmol/L组perilipin A基因mRNA相对表达量极显著高于对照组与0.2 μmol/L组（P<0.01）；1.0 μmol/L组ADRP基因mRNA相对表达量极显著高于其他组（P<0.01）；在48 h时，1.0 μmol/L组甘油释放量、ATGL基因mRNA相对表达量均极显著或显著低于对照组（P<0.01；P<0.05），1.0 μmol/L组perilipin A基因mRNA相对表达量显著高于对照组与0.2 μmol/L组（P<0.05），0.5、1.0 μmol/L组ADRP基因mRNA相对表达量极显著高于对照组（P<0.01）。综上所述，生物素可通过促进perilipin A与ADRP表达，同时抑制ATGL与HSL表达，达到抑制脂肪分解的效果，且浓度为1.0 μmol/L时效果最佳。 |
| 关 键 词： | 生物素 3T3-L1脂肪细胞 脂肪分解 |
| 收稿时间： | 2018-01-22 |
Effects of Different Biotin Levels on Lipolysis and Relevant Genes Expression of 3T3-L1 Adipocytes |
|
| LUO Chenglong,SHAO Wei,REN Wanping,ZHAO Yankun,WU Kaile,YU Xiong.Effects of Different Biotin Levels on Lipolysis and Relevant Genes Expression of 3T3-L1 Adipocytes[J].China Animal Husbandry & Veterinary Medicine,2018,45(7):1783-1790. | |
| Authors: | LUO Chenglong SHAO Wei REN Wanping ZHAO Yankun WU Kaile YU Xiong |
| Institution: | 1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China; 2. Xinjiang Key Laboratory of Meat and Milk Production Herbivore Nutrition, Urumqi 830052, China |
| Abstract: | In order to explore the effects of different biotin levels on lipolysis and relevant gene expression of 3T3-L1 adipocytes,3T3-L1 cells were differentiate into 3T3-L1 adipocytes by the triplet induction method.3T3-L1 adipocytes were treated with 0 (control group),0.2, 0.5 and 1.0 μmol/L biotin upon being concentrated,and relative expression levels of ATGL,HSL,perilipin A and ADRP were measured at 12,24 and 48 h,respectively.The results showed that:At 12 h,the glycerol emission and ATGL gene mRNA relative expression of 1.0 μmol/L group were extremely significantly lower than that of control group (P<0.01).The HSL gene mRNA relative expression of 0.5 and 1.0 μmol/L groups were significantly lower than that of control group (P<0.05),while the mRNA relative expression of perilipin A and ADRP genes in 1.0 μmol/L group were extremely significantly higher than that of control group and 0.2 μmol/L group (P<0.01).At 24 h,the glycerol emission of three test groups and ATGL gene mRNA relative expression of 1.0 μmol/L group were extremely significantly lower than that of control group (P<0.01).The perilipin A gene mRNA relative expression of 0.5 and 1.0 μmol/L groups were extremely significantly higher than that of control group and 0.2 μmol/L group (P<0.01),and the mRNA relative expression of ADRP gene of 1.0 μmol/L group were extremely significantly higher than that of other groups (P<0.01).At 48 h,the glycerol emission and ATGL gene mRNA relative expression of 1.0 μmol/L group were extremely significantly or significatly lower than that of control group (P<0.01;P<0.05).The perilipin A gene mRNA relative expression of 1.0 μmol/L group was significantly higher than that of control group and 0.2 μmol/L group (P<0.05),and the mRNA relative expression of ADRP gene of 0.5 and 1.0 μmol/L groups were extremely significantly higher than that of control group (P<0.01).In conclusion,the biotin could inhibit the lipolysis through promoting the relative expression of perilipin A and ADRP and reducing the relative expression of ATGL and HSL.Moreover,when the concentration was 1.0 μmol/L,the effect was optimal. |
| Keywords: | biotin 3T3-L1 adipocytes lipolysis |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 | |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 | |