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| 猪伪狂犬病病毒天津变异株R13139对猪的致病性研究 | |
| 引用本文： | 任卫科,张莉,李秀丽,池晶晶,田向学,李富强,董志民,鄢明华,仇华吉.猪伪狂犬病病毒天津变异株R13139对猪的致病性研究[J].中国畜牧兽医,2018,45(9):2620-2627. |
| 作者姓名： | 任卫科 张莉 李秀丽 池晶晶 田向学 李富强 董志民 鄢明华 仇华吉 |
| 作者单位： | 1. 农业部兽用药物与诊断技术天津科学观测实验站, 天津 300381; 2. 天津市畜牧兽医研究所, 天津 300381; 3. 中国农业科学院哈尔滨兽医研究所, 哈尔滨 150069 |
| 基金项目： | 天津市自然科学基金项目（15JCYBJC30000）；天津市生猪产业技术体系项目（ITTPRS2017003）；天津市农业科技成果转化与推广项目（201601070）；天津市科技计划项目（14ZXNZNC00047）；中国农业科学院重大平台推进计划（Y2017PT50） |
| 摘 要： | 为了解猪伪狂犬病病毒（PRV）变异株R13139株对猪的致病性情况，本试验将PRV R13139株和PRV经典强毒株SC株均以106 TCID50剂量经滴鼻途径接种6和9周龄健康仔猪各3头，空白对照组鼻腔接种生理盐水，通过攻毒后两个年龄段猪的临床症状表现、眼观病例变化、显微病理变化、抗体产生情况及病毒在体内部分情况评定两毒株对猪的致病力差异。结果显示，SC株对6和9周龄猪的致死率分别为66.7%（2/3）和100.0%（3/3），而R13139株对6和9周龄猪的致死率分别为33.3%（1/3）和66.7%（2/3），表明R13139株对猪的致死性弱于SC株，但R13139株引起两个年龄段猪发病时间比SC株早，同时间段的体温升高幅度更高，神经发症状更严重；眼观病理变化发现，R13139株引起的发病猪肝脏坏死现象比SC株更明显，而心脏、肺脏、脾脏、膀胱和脑的病理变化差异不明显；显微病理结果显示，R13139株对病猪的扁桃体、肺脏、肝脏和三叉神经节等组织造成的病理损伤明显强于SC株；PCR结果显示，R13139株在濒死猪扁桃体、脾脏、肺脏、三叉神经节和淋巴节（腹股沟、肠系膜和颌下）等组织脏器中分布比SC株更广泛；应用ELISA检测攻毒猪抗体发现，R13139株诱导PRV-gB抗体产生的时间比SC株早1 d。本研究结果可为揭示中国伪狂犬病重新流行的原因及进一步开展防控技术研究提供科学依据。 |
| 关 键 词： | 伪狂犬病病毒(PRV) 变异株 致病性 |
| 收稿时间： | 2018-01-31 |
Pathogenicity of Pseudorabies Virus Tianjin Variant Strain R13139 in Pigs |
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| REN Weike,ZHANG Li,LI Xiuli,CHI Jingjing,TIAN Xiangxue,LI Fuqiang,DONG Zhimin,YAN Minghua,QIU Huaji.Pathogenicity of Pseudorabies Virus Tianjin Variant Strain R13139 in Pigs[J].China Animal Husbandry & Veterinary Medicine,2018,45(9):2620-2627. | |
| Authors: | REN Weike ZHANG Li LI Xiuli CHI Jingjing TIAN Xiangxue LI Fuqiang DONG Zhimin YAN Minghua QIU Huaji |
| Institution: | 1. Tianjin Scientific Observation Experiment Station of Veterinary Medicine and Diagnosis Technology, Ministry of Agriculture, Tianjin 300381, China; 2. Tianjin Animal Science and Veterinary Research Institute, Tianjin 300381, China; 3. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China |
| Abstract: | In order to determine the pathogenicity of the pseudorabies virus (PRV) variant R13139 strain in pigs,PRV R13139 strain and PRV classic virulent SC strain were inoculated intranasally with 106 TCID50 to inoculate each of 3 healthy piglets at 6 and 9 weeks of age,and blank control group pigs were intranasally with saline.The pathogenicity difference of the two strains were evaluated by clinical manifestations,gross pathology,histopathology,antibody production and virus distribution in organs of infected pigs.The results showed that the lethality rates of SC strain to 6 and 9 weeks old pigs were 66.7%(2/3) and 100%(3/3) respectively,while the lethality rates of R13139 strain to 6 and 9 weeks old pigs were 33.3%(1/3) and 66.7%(2/3) respectively,indicating that R13139 strain was lower mortality than SC strain to 6 and 9 weeks old pigs;However,compared to SC strain infection,R13139 strain caused an earlier onset of clinical signs,higher temperature rise in the same time period and more serious neurologic symptoms in 6 and 9 weeks old pigs;The gross pathology showed that the liver lesions caused by R13139 strain had more obvious necrosis than those of SC strain;The pathological changes of heart,lung,spleen,bladder and brain were not very different;Histopathological examination revealed that the pathological damage caused by R13139 strain of tonsil,lung,liver and trigeminal ganglia of infected pigs was remarkably obvious than those of SC strain;Meanwhile,the PCR results showed the R13139 strain was more widely distributed in tonsil,spleen,lung,trigeminal ganglion and lymph node (inguinal,mesenteric and submandibular) of dead pig's organ samples;The results of ELISA detection showed the R13139-infected pig's PRV-gB antibodies produced earlier for 1 d than those of the SC-infected pigs.The results could provide data for revealing the causes of the reepidemic of pseudorabies in China and further research on the prevention and control technology. |
| Keywords: | pseudorabies virus (PRV) variant strain pathogenicity |
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