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| 新疆伊犁地区马疱疹病毒1型ORF30基因序列分析 | |
| 引用本文： | 范斌,王燕,阿玛古丽&#,朱马汉,冉多良,刘建华,加尔肯.新疆伊犁地区马疱疹病毒1型ORF30基因序列分析[J].中国畜牧兽医,2018,45(1):65-70. |
| 作者姓名： | 范斌 王燕 阿玛古丽&# 朱马汉 冉多良 刘建华 加尔肯 |
| 作者单位： | 1. 新疆农业大学动物医学学院, 家畜传染病实验室, 乌鲁木齐 830052; 2. 新疆畜牧科学院畜牧业经济与信息研究所, 乌鲁木齐 830011 |
| 基金项目： | 新疆少数民族科技人才特殊培养计划科研项目（2016D0214） |
| 摘 要： | 为研究马疱疹病毒1型（EHV-1-XJ2015）新疆伊犁分离株致病基因型，评估新疆EHV-1-XJ2015的潜在风险因素，本试验设计特异性引物，应用PCR技术扩增EHV-1-XJ2015分离株ORF30基因相应区域，连接至克隆载体，成功构建重组质粒pMD19-T-ORF30。测序结果表明，EHV-1-XJ2015毒株ORF30基因2 254 bp处为A，且编码天冬酰胺（N），分析证明EHV-1-XJ2015基因型为非神经型毒株，毒力倾向表现为流产型毒株；遗传进化显示，EHV-1-XJ2015毒株与日本分离株90c16、00c19、HH1、NY03为同一分支，氨基酸同源性最高为100%。本研究结果为开展EHV-1分子流行病学调查研究及为新疆地区现有诊断方法和防控措施的评估提供理论依据。 |
| 关 键 词： | EHV-1 ORF30 致病基因型 序列比对 |
| 收稿时间： | 2017-08-03 |
Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang |
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| FAN Bin,WANG Yan,Zhumahan&#,A,RAN Duoliang,LIU Jianhua,Jarhen.Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang[J].China Animal Husbandry & Veterinary Medicine,2018,45(1):65-70. | |
| Authors: | FAN Bin WANG Yan Zhumahan&# A RAN Duoliang LIU Jianhua Jarhen |
| Institution: | 1. Laboratory of Animal Infectious Disease, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China; 2. Xinjiang Academy of Animal Husbandry Economics and Information Research Institute, Urumqi 830011, China |
| Abstract: | To study the genotypes of EHV-1-XJ2015 strain isolated from Ili area of Xinjiang, this study investigated the potential risk factors for EHV-1-XJ2015.In this experiment, specific primers was designed and using PCR technique to amplify corresponding region of EHV-1-XJ2015 isolate ORF30 gene, connected to vector pMD19-T,and the recombinant plasmid pMD19-T-ORF30 was successfully constructed. Sequencing results showed that EHV-1-XJ2015 strain ORF30 gene 2 254 bp site was A, and encoding asparagine (N).It was proved that EHV-1-XJ2015 was a non-neurotoxic strain, virulent strains tend to behave as a miscarriage. The phylogenetic analysis showed that the EHV-1-XJ2015 strain was the same branch as the 90c16, 00c19, HH1, NY03 isolated from Japan, amino acid homology was the highest and reached 100%. The results provided a theoretical basis for the study of EHV-1 molecular epidemiology and the assessment of existing diagnostic methods and prevention and control measures in Xinjiang, and ultimately achieved the purpose of prevention and control of the disease. |
| Keywords: | EHV-1 ORF30 pathogenicity genotype sequence alignment |
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