Nodosin induces apoptosis of HepG2 cells by increasing Apaf-1 expression and activating caspase-3 |
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| Authors: | HAI Guang-fan ZHANG Hui MA Jing GUO Lan-qing WANG Hai-yan |
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| Institution: | 1. Pharmacy School, Xinxiang Medical University, Xinxiang 453003, China;
2. The Second Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, China;
3. The Nursing School, Xinxiang Medical University, Xinxiang 453003, China;
4. The First Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, China |
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| Abstract: | AIM:To investigate the effects of nodosin on apoptosis of human hepatocellular carcinoma HepG2 cells. METHODS:HepG2 cells were treated with nodosin at different concentrations (1.25 μmol/L, 2.5 μmol/L, 5 μmol/L, 10 μmol/L and 20 μmol/L) for 24 h. The morphological changes of the HepG2 cells were observed by Hoechst 33258 staining and electron microscopy. The apoptotic rates were analyzed by flow cytometry. The mRNA expression of apoptotic protease-activating factor-1 (Apaf-1) was detected by RT-qPCR. The protein levels of pro-caspase-3, caspase-3 and cleaved caspase-3 were determined by Western blot. RESULTS:HepG2 cells showed obvious cell shrinkage and nucleus drift when treated with nodosin as the concentration was increased. Many apoptotic bodies were observed in 5 μmol/L, 10 μmol/L and 20 μmol/L nodosin groups. The mRNA expression of Apaf-1 was increased in 5 μmol/L, 10 μmol/L and 20 μmol/L nodosin groups as compared with control group (P<0.05). The protein levels of pro-caspase-3, caspase-3 and cleaved caspase-3 were increased with the increasing dose of nodosin (P<0.05). CONCLUSION:Nodosin induces the apoptosis of HepG2 cells. This effect was related to increasing Apaf-1 mRNA expression and subsequently promoting the activation of caspase-3. |
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| Keywords: | Hepatocellular carcinoma Nodosin Apoptosis Caspase-3 Apoptotic protease-activating factor-1 |
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