| 致病性鹅源新城疫病毒对鸡胚成纤维细胞的致病变特性 |
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| 引用本文： | 万洪全,卢军,刘秀梵,陈平,陈素娟,冀德君.致病性鹅源新城疫病毒对鸡胚成纤维细胞的致病变特性[J].动物医学进展,2004,25(5):89-91. |
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| 作者姓名： | 万洪全 卢军 刘秀梵 陈平 陈素娟 冀德君 |
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| 作者单位： | 扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
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| 基金项目： | "973"项目(G19990199)
江苏省自然科学基金青年科技创新人才项目(BK2003403)
江苏省高校自然科学研究计划项目(03KJB230158)
扬州大学高层次人才科研启动基金项目 |
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| 摘 要： | 选择4株鹅源新城疫病毒(NDV)接种鸡胚成纤维细胞(CEF)培养物,研究鹅源NDV对CEF的致病变特性,并与鸡源及鸽源NDV进行比较。结果显示,鹅源NDV接种次代CEF后24h开始有病变产生,表现为少数细胞变圆、皱缩,折光性增强,随后病变缓慢进展,感染细胞形成大量合胞体,到84～144h时,CEF单层彻底破坏;鸡源和鸽源MDV接种CEF后也在24h左右开始有病变产生,最初病变与鹅源毒株导致的病变相似,但病变进展迅速,细胞显著裂解并导致单层的严重破坏,60～84h时细胞单层即彻底破坏。对病毒接种后不同时间段培养物上清血凝(HA)效价的测定结果表明,鹅源NDV接种后84～120h培养物上清HA效价达到高峰,为5～8 log2,鸡源和鸽源NDV接种后60～84h HA效价即可达到高峰,但其最高效价只有4 log2。
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| 关 键 词： | 鹅 新城疫病毒 鸡胚成纤维细胞 病变 |
| 文章编号： | 1007-5038(2004)05-0089-03 |
| 修稿时间： | 2004年4月20日 |
Cytopathic Alterations of Chicken Embryo Fibroblasts Induced by Newcastle disease virus of Goose Origin |
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| WAN Hong-quan,LU Jun,LIU Xiu-fan,CHEN Ping,CHEN Su-juan,JI De-jun.Cytopathic Alterations of Chicken Embryo Fibroblasts Induced by Newcastle disease virus of Goose Origin[J].Progress In Veterinary Medicine,2004,25(5):89-91. |
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| Authors: | WAN Hong-quan LU Jun LIU Xiu-fan CHEN Ping CHEN Su-juan JI De-jun |
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| Abstract: | The cytopathic effects on chicken embryo fibroblasts (CEFs), induced by Newcastle disease virus (NDV) derived from the novel disease outbreaks in geese during 1997 - 2000, were compared with those induced by chicken and pigeon isolates. When the CEFs were inoculated with goose isolates, cell abnormalities could be seen as early as 24 h PI, characterized by occurrence of some rounded,refractile and dark appearing cells. Then the lesions became more severe gradually, with a large number of syncytial cells appeared. The CEF monolayers were destroyed completely at 84-144 h PI, and the HA titers in the culture supernatants could get as high as 5-8 log2. When infected with ND viruses of chicken or pigeon origin, the earliest cell lesions were observed at 24 h PI, too. But it appeared that the alterations developed more rapidly in this occasion. The cells became rounded and refractile and necrosized, leaving empty areas (holes) in the cell sheet. At 60-84 h PI, the monolayers destroyed, with HA titers of 3-4 log2 in the culture supernatant. The syncytial cells contained far less nuclei than those formed after inoculation of goose isolates. |
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| Keywords: | goose Newcastle disease virus chicken embryo fibroblasts Cytopathic alterations |
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