| 利用根系分泌物与绿色荧光蛋白标记的病原菌互作关系鉴定香蕉对枯萎病的抗性 |
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| 引用本文： | 左存武,孙清明,黄秉智,李春雨,易干军.利用根系分泌物与绿色荧光蛋白标记的病原菌互作关系鉴定香蕉对枯萎病的抗性[J].园艺学报,2010,37(5):713-720. |
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| 作者姓名： | 左存武 孙清明 黄秉智 李春雨 易干军 |
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| 作者单位： | （1广东省农业科学院果树研究所，广州510640；2江西农业大学农学院，南昌330045） |
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| 基金项目： | 国家自然科学基金项目(30971991); 国家科技攻关计划(2008BAD92B06); 农业部‘948’项目(2008-G1) |
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| 摘 要： | 利用PEG-CaCl2法将携带绿色荧光蛋白（sGFP）的表达载体pCT74转化进香蕉枯萎病菌4号生理小种（CGMCCC 3.12196），sGFP在病原菌的菌丝及孢子的细胞质和细胞核内稳定表达。将sGFP标记的病原菌接种巴西蕉，证明sGFP的导入未引起病原菌致病性发生改变。水培9个枯萎病抗性级别不同的香蕉品种，分别收集根系分泌物，生物测定其对sGFP标记的病原菌的抑菌作用。荧光显微镜观察结果表明：高抗枯萎病的品种根系分泌物的有机成分对病原菌孢子有致死作用，中抗品种的对病原菌孢子萌发、菌丝的生长有显著抑制作用，相反感病品种的根系分泌物则能够起促进作用。
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| 关 键 词： | 香蕉 香蕉枯萎病 绿色荧光蛋白 根系分泌物 抗病性鉴定 |
| 收稿时间： | 2009-12-20 |
Screening Method for Resistance to Fusarium Wilt of Banana Basing on Green Fluorescent Protein Tagged Pathogen and Root Exudates |
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| ZUO Cun-wu,SUN Qing-ming,HUANG Bing-zhi,LI Chun-yu,YI Gan-jun.Screening Method for Resistance to Fusarium Wilt of Banana Basing on Green Fluorescent Protein Tagged Pathogen and Root Exudates[J].Acta Horticulturae Sinica,2010,37(5):713-720. |
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| Authors: | ZUO Cun-wu SUN Qing-ming HUANG Bing-zhi LI Chun-yu YI Gan-jun |
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| Institution: | （1Institution of Fruit Tree Research，Guangdong Academy of Agricultural Sciences，Guangzhou 510640，China；2College of Agronomy，Jiangxi Agricultural University，Nanchang 330045，China） |
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| Abstract: | A green fluorescent protein (sGFP) tagged Foc4 strain was developed by the method of PEG-CaCl2.The fluorescent signal was clearly visible in cytoplasm and nucleus of the hyphae and spores under the fluorescence microscopy.The transformed Foc retained its pathogenicity and growth patterns.Water culture of 9 banana cultivars with different resistance were performed for 15 days using 200 mL of sterile distilled water for each plantlet,and the root exudates were collected and bioassay for their effects on the G... |
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| Keywords: | banana fusarium wilt of banana green fluorescent protein root exudates bioassay of resistance |
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