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孜然根腐病病原分离及鉴定
引用本文:刘淑娟,陈秀蓉,袁宏波.孜然根腐病病原分离及鉴定[J].浙江农业学报,2012,24(3):481-485.
作者姓名:刘淑娟  陈秀蓉  袁宏波
作者单位:1. 甘肃省荒漠化与风沙灾害防治重点实验室—省部共建国家重点实验室培育基地甘肃省治沙研究所,甘肃兰州730070;甘肃农业大学草业学院,甘肃兰州730070
2. 甘肃农业大学草业学院,甘肃兰州,730070
3. 甘肃省荒漠化与风沙灾害防治重点实验室—省部共建国家重点实验室培育基地甘肃省治沙研究所,甘肃兰州,730070
基金项目:甘肃省农业综合开发项目(NFGSND0805);国家自然基金项目(31160116)
摘    要:选取孜根腐病病株根系分离、鉴定出Fusarium oxysporum Schlecht,Fusarium solani(mart.)Sacc.和Rhizoctonia sp.和未知真菌(属于半知菌亚门,种属待定)4种主要致病菌.致病性测定结果表明:4种菌表现出不同程度的致病性,未知真菌单独接种发病率为70.0%,致病性强,是孜然根腐病的主要致病菌;其次为F.oxysporum和F.solani,发病率分别为50.9%和43.2%;Rhizoctonia sp.的发病率较低,为11.0%.用未知真菌、F.oxysporum、F.solan和Rhizoctonia sp.进行混和接种,其发病率为97.4%,症状典型,说明孜然根腐病由多种病原菌复合侵染所致.

关 键 词:孜然  根腐病  茄腐镰孢菌  尖镰孢菌  丝核菌

Identification and isolation of root rot pathogen of Cucuminum cyminum L.
LIU Shu-juan , CHEN Xiu-rong , YUAN Hong-bo.Identification and isolation of root rot pathogen of Cucuminum cyminum L.[J].Acta Agriculturae Zhejiangensis,2012,24(3):481-485.
Authors:LIU Shu-juan  CHEN Xiu-rong  YUAN Hong-bo
Institution:1(1State Key Laboratory of Desertification and Aeolian Sand Disaster Combating,Gansu Desert Control Research Institute,Lanzhou 730070,China;2 Pratacultural College,Gansu Agriculture University,Lanzhou 730070,China)
Abstract:Diseased roots of root rot were selected and pathogen of Cucuminum cyminum L.,Fusarium oxysporum,Fusarium solani(mart.) Sacc.,Rhizoctonia sp.and a kind of unknown pathogen belonged to Deuteromycotina were isolated and identified.After the wound inoculation,the incidence of root rot diseases of a kind of unknown pathogen,which belonged to Deuteromycotina,showed a higher value of 70.0%,F.oxysporum,F.solani and Rhizoctonia were 50.9%,43.2%and 11.0%,respectively.The incidence of root rot diseases of its mixture inoculation was 97.4%.The test of isolation and identification of the pathogen and pathogenecity showed that the root rot of Cucuminum cyminum L.was caused by a complex infection of F.oxysporum,F.solani,Rhizoctonia and a kind of unknown pathogen belonged to Deuteromycotina.
Keywords:Cucuminum cyminum L  root rot  Fusarium oxysporum Schlecht  Fusarium solani(mart  )Sacc    Rhizoctonia sp  
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