| 玉米粉水解过程中还原糖的测定 |
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| 引用本文： | 于鲁浩,马耀宏,杨俊慧,孟庆军,杨艳.玉米粉水解过程中还原糖的测定[J].现代农业科技,2011(17):33-34. |
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| 作者姓名： | 于鲁浩 马耀宏 杨俊慧 孟庆军 杨艳 |
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| 作者单位： | 1. 山东轻工业学院食品与生物工程学院,山东济南,250353
2. 山东省科学院生物研究所,山东省生物传感器重点实验室 |
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| 摘 要： | 为了测定玉米粉水解过程中还原糖的含量,将玉米粉在水浴中进行酸水解,加入的玉米粉、蒸馏水和稀硫酸的比例是1∶100∶15,温度保持在100℃。在水解的过程中,采用还原糖测定仪法和DNS法(3,5-二硝基水杨酸法)测定水解过程中的还原糖量,每1 h测定1次,从而准确地了解玉米粉的水解程度。结果表明:2种方法都比较适宜测定还原糖含量,但还原糖测定仪法更具优势,其稳定性好,重现性好,测定速度快,准确度高。
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| 关 键 词： | 玉米粉 还原糖测定仪法 DNS法 还原糖 |
Determination of Reducing Sugar in Corn Flour Hydrolysis |
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| Authors: | YU Lu-hao MA Yao-hong YANG Jun-hui MENG Qing-jun YANG Yan |
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| Institution: | YU Lu-hao 1 MA Yao-hong 2 YANG Jun-hui 2 MENG Qing-jun 2 YANG Yan 2(1 School of Food & Bioengineering,Shandong Polytechnic University,Jinan Shandong 250353,2 Key Laboratory for Biosensors of Shandong Province,Biology Institute of Shandong Academy of Sciences) |
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| Abstract: | To determine the reducing sugar in corn flour hrdrolysis ,corn flour was hydrolyzed by dilute sulphuric acid in water-bath ,the ratio of the quantity of corn,distilled water and dilute sulphuric acid added was 1：100：15,and the temperature was remained at 100 ~C .In the hydrolyzing process, reducing sugar content was determined by reducing sugar analyzer and DNS method per hour, so the hydrolyzing level was known exactly.The results showed that two methods were both suitable to determine the reducing sugar content, but the reducing sugar analyzer had more advantageous. This method was stable and with good reproducibility, it characterized by speediness and high accuracy. |
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| Keywords: | corn flour reducing sugar analyzer DNS reducing sugar |
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