| 京郊设施蔬菜黄瓜花叶病毒的检测 |
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| 引用本文： | 陈 柳,邢冬梅,刘正雄,陈笑瑜,杨瑞,杨建强,胡学军,尚巧霞.京郊设施蔬菜黄瓜花叶病毒的检测[J].中国农学通报,2015,31(20):82-85. |
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| 作者姓名： | 陈 柳 邢冬梅 刘正雄 陈笑瑜 杨瑞 杨建强 胡学军 尚巧霞 |
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| 作者单位： | (;1.农业应用新技术北京市重点实验室/北京农学院植物科学技术学院,北京 102206；;2.北京市顺义区牛栏山第二小学,北京 101301；;3.北京市昌平区植保植检站,北京 102200；;4.北京市植物保护站,北京 100029） |
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| 基金项目： | 基金项目：北京市属高等学校高层次人才引进与培养三年行动计划“青年拔尖人才培育计划”(CIT&TCD201304091)；北京农学院研究生改革与发展项目“全日制农业推广硕士人才培养模式研究与实践”(2014YJS003)；北京农学院研究生改革与发展项目“植物保护领域专业学位研究生培养机制探索”(2014YJS004)；北京农学院研究生改革与发展项目“植物保护一级学科学术学位授权点培育”(2014YJS005)。 |
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| 摘 要： | 为了明确北京地区设施蔬菜上常见病毒的发生情况，利用ELISA和RT-PCR方法对京郊设施蔬菜中的黄瓜花叶病毒(Cucumber mosaic virus，CMV)进行检测。共采集设施栽培的芹菜、观赏南瓜、结球生菜、大南瓜、辣椒、抱子甘蓝、番茄（‘大红袍’和‘红罗马’品种）、四棱豆、茄子共10个蔬菜作物样品的叶片进行测定。结果显示：芹菜、观赏南瓜、结球生菜、辣椒、茄子和番茄‘大红袍’品种的样品含有CMV，大南瓜、抱子甘蓝、四棱豆和番茄‘红罗马’品种的样品未检测到CMV。ELISA和RT-PCR检测结果一致，将检测结果为阳性样品的RT-PCR产物进行了序列测定和分析，验证了检测结果的可靠性。
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| 关 键 词： | 聚类 聚类 |
| 收稿时间： | 2014/9/13 0:00:00 |
| 修稿时间： | 7/6/2015 12:00:00 AM |
Detection of Cucumber Mosaic Virus Infecting Vegetable Crops Grown in Beijing Suburban Greenhouses |
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| Chen Liu,Xing Dongmei,Liu Zhengxiong,Chen Xiaoyu,Yang Jianqiang,Hu Xuejun and Shang Qiaoxia.Detection of Cucumber Mosaic Virus Infecting Vegetable Crops Grown in Beijing Suburban Greenhouses[J].Chinese Agricultural Science Bulletin,2015,31(20):82-85. |
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| Authors: | Chen Liu Xing Dongmei Liu Zhengxiong Chen Xiaoyu Yang Jianqiang Hu Xuejun and Shang Qiaoxia |
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| Institution: | (;1.Beijing Key Laboratory of New Technology in Agricultural Application/ College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206;2.Niulanshan Second Primary School of Shunyi District, Beijing 101301;3.Plant Protection and Quarantine Station of Changping District, Beijing 102200;4.Beijing Plant Protection Station, Beijing 100029) |
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| Abstract: | Cucumber mosaic virus (CMV) was detected by ELISA and RT-PCR methods to investigate the distribution and diversity of viruses infecting vegetable crops grown in Beijing suburban greenhouses. Leave samples of 10 vegetable crops were collected and then tested. Identical results were obtained by both ELISA and RT-PCR methods showed that 6 samples of celery, ornamental pumpkin, lettuce, peppers, eggplant and tomato ‘Dahongpao’ were positive while the other 4 samples of great pumpkin, brussels sprouts, four winged bean and tomato ‘Rome red’ were negative to CMV. In our study, the reliability of the results was confirmed by sequencing and sequence analysis of the RT-PCR products from the positive samples. |
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| Keywords: | vegetable crops in greenhouse cucumber mosaic virus ELISA RT-PCR detection |
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