| 抑制油葵花药培养褐变的研究初报 |
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| 引用本文： | 王梦员,侯建华,于海峰.抑制油葵花药培养褐变的研究初报[J].辽宁农业科学,2008(3):12-14. |
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| 作者姓名： | 王梦员 侯建华 于海峰 |
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| 作者单位： | 1. 内蒙古农业大学农学院,内蒙古,呼和浩特,010019
2. 内蒙古农牧科学院作物研究所,内蒙古,呼和浩特,010031 |
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| 基金项目： | 内蒙古自然科学基金，引进国际先进农业科技计划(948计划) |
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| 摘 要： | 以油葵的花药为外植体,在附加不同浓度激素的MS培养基中添加Vc(抗坏血酸)、AC(活性碳)、PVP(聚乙烯吡咯烷酮)对油葵花药愈伤组织进行抑制褐变处理.结果表明,AC在抑制褐变的同时也抑制了愈伤组织的生长,300 mg/L Vc及0.1%PVP均可有效抑制褐变,其中0.1%PVP抑制褐变的效果优于300 mg/L Vc.
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| 关 键 词： | 油葵 花药培养 褐变 |
Preliminary Study on Inhibition of Browning in Anther Culture of Oil Sunflower |
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| WANG Meng-yuan,HOU Jian-hua,YU Hai-feng.Preliminary Study on Inhibition of Browning in Anther Culture of Oil Sunflower[J].Liaoning Agricultural Sciences,2008(3):12-14. |
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| Authors: | WANG Meng-yuan HOU Jian-hua YU Hai-feng |
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| Abstract: | The different concentrations of Vc(Vitamin C),AC(Activated charcoal) and PVP(Polyvinylpyrrolidone) were used in the medium to inhibite the browning of anther culture.The effect of Ac not only inhibited browning but also the growth of callus.The browning was inhibited effectively by 300 mg/L Vc and 0.1%PVP.And 0.1%PVP had better effect than 300 mg/L Vc. |
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| Keywords: | Helianthus annuus Anther culture Browning |
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