| 聚合酶链反应（PCR）检测养殖对虾的白斑症病毒（WSSV）感染 |
| |
| 引用本文： | 战文斌,邢婧,王远红,铃木信一,福田颖穗.聚合酶链反应（PCR）检测养殖对虾的白斑症病毒（WSSV）感染[J].中国水产科学,2000,7(1). |
| |
| 作者姓名： | 战文斌 邢婧 王远红 铃木信一 福田颖穗 |
| |
| 作者单位： | 教育部水产养殖重点实验室青岛海洋大学!山东青岛266003(战文斌，邢婧，王远红)，水族病理研究室东京水产大学!日本(铃木信一，福田颖穗) |
| |
| 基金项目： | 8 63海洋领域海洋生物技术资助项目! (819-Q - 0 8)，国家重点基础研究规划项目! (G19990 12 0 0 2 )，教育部留学回国人员
科研启动 |
| |
| 摘 要： | 对虾WSSV病是亚洲对虾养殖业中的一个棘手问题。本研究采用Kimura引物 ,用PCR技术对不同生长期的中国对虾 (Penaeuschinensis)进行了WSSV的检测 ,同时也检测了对虾发病时养殖池中多见的野生厚蟹 (Helicesp .)和矛尾刺虎鱼 (Acanthogobiushasta)。检测结果表明 :分别在检测的 5尾亲虾中的 1尾 ,6尾仔虾中的 1尾 ,5尾稚虾中的 3尾及所检测的 5尾病虾和 2只厚蟹中获得到 982bp的PCR扩增产物 ,说明为WSSV感染阳性。在检测的 2尾矛尾刺虎鱼中均未获得PCR扩增产物 ,说明为WSSV感染阴性。在亲虾、虾苗以及虾池内的野生厚蟹中检测到WSSV感染的阳性结果表明 :WSSV感染的亲虾有可能是病毒的储主 ,WSSV感染的野生厚蟹有可能是病毒中间宿主或病毒的携带者 ,它们在对虾WSSV病的感染、传播中起了重要的作用
|
| 关 键 词： | 对虾 白斑症病毒 聚合酶链反应 |
| 修稿时间： | 2010/4/8 0:00:00 |
Detection of white spot syndrome virus (WSSV) in cultured shrimp by PCR |
| |
| ZHAN Wen bin ,XING Jing ,WANG Yuan hong ,Suziki S ,Fukuda H.Detection of white spot syndrome virus (WSSV) in cultured shrimp by PCR[J].Journal of Fishery Sciences of China,2000,7(1). |
| |
| Authors: | ZHAN Wen bin XING Jing WANG Yuan hong Suziki S Fukuda H |
| |
| Institution: | ZHAN Wen bin 1,XING Jing 1,WANG Yuan hong 1,Suziki S 2,Fukuda H 2 |
| |
| Abstract: | White spot syndrome virus (WSSV) disease is a key problem in shrimp culture industry in Asian countries. The present study attempted to detect the WSSV infected in some developmental stage of Penaeus chinensis by PCR using Kimura primer. And wild crab (Helice sp.) and javelin goby (Acanthogobius hasta) captured from shrimp culture pond were also detected. The detecting results showed that one out of five shrimp spawners, one out of six postlarvae, three out of five juveniles, all of five diseased shrimps and all of two crabs yielded a 982 bp PCR product while two javelin goby no PCR product appeared. WSSV positive was obtained from the spawners, seedings and wild crabs in shrimp culture ponds. It was indicated that WSSV-infected spawners maybe the virus reservoir and the wild crabs maybe the virus inter-host or carrier probably. They play an important role in transmission and spreading of shrimp WSSV disease. |
| |
| Keywords: | Penaeus chinensis WSSV PCR |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
