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| 3种方法检测黄瓜绿斑驳花叶病毒（CGMMV）的灵敏度对比分析 | |
| 引用本文： | 李海明,沈建国,吴祖建,陈启建.3种方法检测黄瓜绿斑驳花叶病毒（CGMMV）的灵敏度对比分析[J].中国农学通报,2010,26(17):269-272. |
| 作者姓名： | 李海明 沈建国 吴祖建 陈启建 |
| 作者单位： | 1. 福建农林大学植物病毒研究所,福州,350002;福建省农科院甘蔗研究所,福建漳州,363005 2. 福建出入境检验检疫局,福州,350001 3. 福建农林大学植物病毒研究所,福州,350002 |
| 基金项目： | 国家自然科学基金项目"鸦胆子素D抗烟草花叶病毒机制研究"，福建省科技重大专项"桔小实蝇和红火蚁的预警与监测技术研究"，福建出入境检验检疫局科技项目"进境果蔬上黄瓜绿斑驳花叶病毒分子检测技术的研究" |
| 摘 要： | 本研究以黄瓜绿斑驳花叶病毒侵染的黄瓜叶片为材料，应用双抗体夹心酶联免疫吸附测定法（DAS-ELISA）、反转录-聚合酶链反应（RT-PCR）、核酸斑点杂交（NASH）技术，进行了检测灵敏度比较研究。三种检测方法灵敏度实验结果表明：RT-PCR检测灵敏度高于地高辛标记的核酸斑点杂交技术，以血清学为基础的DAS-ELISA检测灵敏度最低。通过灵敏度对比分析得出，核酸斑点杂交技术可以替代RT-PCR应用于基层的检疫检测。 |
| 关 键 词： | 控释BB肥 控释BB肥 脲酶 中性磷酸酶 蔗糖酶 |
| 收稿时间： | 2010/3/22 0:00:00 |
| 修稿时间： | 2010/4/29 0:00:00 |
Analysis on comparison of sensitivities of three detecting techniques of Cucumber Green Mottle Mosaic Virus |
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| Li Haiming,Sheng Jianguo,Wu Zujian,Cheng Qijian.Analysis on comparison of sensitivities of three detecting techniques of Cucumber Green Mottle Mosaic Virus[J].Chinese Agricultural Science Bulletin,2010,26(17):269-272. | |
| Authors: | Li Haiming Sheng Jianguo Wu Zujian Cheng Qijian |
| Institution: | Li Haiming, ShengJianguo, Wu Zujian, Cheng Qijian (1Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002; 2Sugarcane Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou Fujian 363005; 3Fujian Exit-Entry Inspection and Quarantine Bureau, Fuzhou 350001) |
| Abstract: | Cucumber green mottle mosaic virus in virus-infected cucumber leaves was detected by DAS-ELISA、RT-PCR and NASH techniques and the sensitivities of detection were compared after the total RNA extracted from 0.1 g virus-infected leaves were diluted gradient 。The minimum weight of virus-infected cucumber leaves detected by DAS-ELISA, RT-PCR and NASH were 39.6 μg, 0.25 ng and 5 ng respectively. The results showed that the detection of RT-PCR was the most sensitive; NASH was more sensitive than DAS-ELISA. NASH technique can be replaced of RT-PCR used in metrical quarantine departments by comparison of sensitivities of three detect techniques. |
| Keywords: | Cucumber green mottle mosaic virus RT-PCR DAS-ELISA NASH comparison of detecting techniques |
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