Genetic mapping of AFLP markers in Japanese bunching onion (<Emphasis Type="Italic">Allium fistulosum</Emphasis>) |
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| Authors: | Email author" target="_blank">Takayoshi?OharaEmail author Yeon-Sang?Song Hikaru?Tsukazaki Tadayuki?Wako Tsukasa?Nunome Akio?Kojima |
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| Institution: | (1) National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Bio-oriented Research Organization (NARO), 360 Kusawa, Ano, Mie 514-2392, Japan;(2) Present address: Mokpo Experiment Station, National Institute of Crop Science, 293-5 Cheongcheon, Cheonggye, Muan, Jeonnam, 534-833, Korea |
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| Abstract: | Summary The first genetic linkage map of Japanese bunching onion (Allium fistulosum) based primarily on AFLP markers was constructed using reciprocally backcrossed progenies. They were 120 plants each of (P1)BC1 and (P2)BC1 populations derived from a cross between single plants of two inbred lines: D1s-15s-22 (P1) and J1s-14s-20 (P2). Based on the (P2)BC1 population, a linkage map of P1 was constructed. It comprises 164 markers – 149 amplified fragment length polymorphisms (AFLPs), 2 cleaved amplified polymorphic
sequences (CAPSs), and 12 simple sequence repeats (SSRs) from Japanese bunching onion, and 1 SSR from bulb onion (A. cepa) – on 15 linkage groups covering 947 centiMorgans (cM). The linkage map of P2 was constructed with the (P1)BC1 population and composed of 120 loci – 105 AFLPs, 1 CAPS, and 13 SSRs developed from Japanese bunching onion and 1 SSR from
bulb onion – on 14 linkage groups covering 775 cM. Both maps were not saturated but were considered to cover the majority
of the genome. Nine linkage groups in P2 map were connected with their counterparts in P1 map using co-dominant anchor markers, 13 SSRs and 1 CAPS. |
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| Keywords: | AFLP Allium fistulosum Japanese bunching onion linkage map SSR |
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