| RAPD标记构建芥蓝×甘蓝分子标记连锁图 |
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| 引用本文： | 陈书霞,王晓武,方智远,程智慧,孙培田.RAPD标记构建芥蓝×甘蓝分子标记连锁图[J].园艺学报,2002,29(3):229-232. |
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| 作者姓名： | 陈书霞 王晓武 方智远 程智慧 孙培田 |
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| 作者单位： | ( 西北农林科技大学园艺系, 杨凌712100; 中国农业科学院蔬菜花卉研究所, 北京100081) |
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| 基金项目： | 农业部“蔬菜遗传与生理重点实验室”资助课题 |
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| 摘 要： | 以芥蓝C100-12×甘蓝秋50-Y7为杂交组合, 构建了F2 作图群体, 利用300 个随机引物对两亲本进行了RAPD 检测, 共筛选到150 个RAPD 引物在亲本间表现多态, 多态引物的比率为58. 3 %。共分析了52个引物产生的96 个多态性RAPD 标记在该群体中的遗传。96 个RAPD 标记中有94 个在F2 群体中的分离比符合3∶1 , 说明该群体适合于QTL 分析, 共发现两对共分离的RAPD 标记。利用该作图群体构建了甘蓝的基本遗传图谱。该图谱由9 个主要的连锁图构成, 覆盖基因总长度约为555. 7 cM。
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| 关 键 词： | 分子标记 连锁图 RAPD 甘蓝 |
| 文章编号： | 0513-353X(2002)03-0229-04 |
| 修稿时间： | 2001年8月2日 |
Construction of Molecular Linkage Map of Brassica oleracea var. alboglabra × B. oleracea var. capitata by RAPD analysis |
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| Chen Shuxia ,Wang Xiaowu ,Fang Zhiyuan ,Cheng Zhihui ,and Sun Peitian.Construction of Molecular Linkage Map of Brassica oleracea var. alboglabra × B. oleracea var. capitata by RAPD analysis[J].Acta Horticulturae Sinica,2002,29(3):229-232. |
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| Authors: | Chen Shuxia Wang Xiaowu Fang Zhiyuan Cheng Zhihui and Sun Peitian |
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| Institution: | ( Department of Horticulture, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | The hybrids were produced by a cross between Brassica oleracea var. capitata and B . oleracea var. alboglabra. A total of 300 random primers were initially screened using two parents , of which 150 showed polymorphism between two parents. The ratio of polymorphism primers is 58. 3 %. 96 F2 individuals were analyzed using 52 10 bp random primers to select polymorphic , stable and repeatable fragment in preliminary segregationanalysis. Most (95. 8 %) of the segregating fragments followed the expected 3∶1 Mendelian segregation ; 4. 2 % showed significant segregation distortion. This shows that the population is suited for analysis of QTLs. There were two pairs of RAPD markers which showed cosegregation. A genetic linkage map of B. oleracea was constructed based on the segregation of 96 RAPD polymorphic loci in one F2 population of B. oleracea var. capitata ×B.oleracea var. alboglabra. The genetic markers defined nine linkage groups, covering 555. 7 recombination units. |
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| Keywords: | Molecular linkage map RAPD Brassica oleracea var capitata |
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