| 离体条件下盐胁迫对马铃薯试管苗叶绿素含量,脯氨酸累积和抗氧化酶活性的影响 |
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| 引用本文： | 李会珍,张志军,许玲,宋文坚,周伟军.离体条件下盐胁迫对马铃薯试管苗叶绿素含量,脯氨酸累积和抗氧化酶活性的影响[J].浙江大学学报(农业与生命科学版),2006,32(3):300-306. |
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| 作者姓名： | 李会珍 张志军 许玲 宋文坚 周伟军 |
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| 作者单位： | 1. 浙江大学,农学系,浙江,杭州,310029;中北大学,环境与安全工程系,山西,太原,030051
2. 浙江大学,农学系,浙江,杭州,310029 |
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| 基金项目： | 中国科学院资助项目;浙江省自然科学基金 |
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| 摘 要： | 离体条件下,研究了2个马铃薯品种在0～80 mmol NaCl胁迫下的试管苗生长、叶绿素含量、脯氨酸和丙二醛含量及氧化酶活性的反应.2品种苗高随盐浓度增加及培养时间的延长而显著降低.与品种紫花白相比,同一盐浓度下静石2号在苗鲜重、根长和根数等方面受抑制程度更强.盐胁迫培养3周后,试管苗叶片中叶绿素a,b与总量及类胡萝卜素含量在40～80 mmol高盐浓度下显著降低.两个品种试管苗中脯氨酸和丙二醛含量随盐浓度的增加而增加,而静石2号中脯氨酸和丙二醛的累积量和累积率都高于紫花白,表明在盐胁迫下静石2号膜脂过氧化程度相对较高.2品种SOD和POD活性均在低盐浓度(20 mmol)下显著增加而高盐浓度下降低.离体条件下,紫花白试管苗呈现出比静石2号高的SOD、POD活性以及较强的耐盐能力,表明其具有进一步在田间进行耐盐基因型筛选的必要性.
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| 关 键 词： | 叶绿素 MDA POD 脯氨酸 盐 马铃薯 SOD |
| 文章编号： | 1008-9209(2006)03-0300-07 |
| 修稿时间： | 2004年5月10日 |
Effect of salinity on chlorophyll contents, proline accumulation and antioxidant enzyme activities of plantlets in vitro in potato |
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| LI Hui-zhen,ZHANG Zhi-jun,XU Ling,SONG Wen-jian,ZHOU Wei-jun.Effect of salinity on chlorophyll contents, proline accumulation and antioxidant enzyme activities of plantlets in vitro in potato[J].Journal of Zhejiang University(Agriculture & Life Sciences),2006,32(3):300-306. |
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| Authors: | LI Hui-zhen ZHANG Zhi-jun XU Ling SONG Wen-jian ZHOU Wei-jun |
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| Abstract: | Responses of plantlets of two potato cultivars to NaCl stress at 0-80 mmol concentration were investigated under in vitro conditions in terms of shoot growth,chlorophyll,proline and malondialdehyde(MDA) contents,and antioxidant enzyme activities.With the increase of NaCl concentrations and the time of exposure ,the shoot length decreased significantly for two cultivars tested.Compared to cultivar Zhihuabai,Jingshi-2,plantlets were more inhibited in terms of shoot,fresh weight,root length and number of roots.After 3 weeks of salt stress,the content of chlorophyll a,chlorophyll b and total chlorophyll,and carotenoid of shoot in plantlets were reduced significantly at higher NaCl concentrations(40-80 mmol).Salinity applied from 20 to 80 mmol progressively increased proline and MDA levels in shoot of both cultivars.The amount and rate of accumulations of MDA and proline were higher in cultivar Jingshi-2 than Zihuabai, suggesting a higher degree of lipid peroxidation in Jingshi-2 due to salt stress,low salinity level(20 mmol) led to a significant increase in superoxide dismutase(SOD) and peroxidase(POD) activities but high concentration made them decreased in both cultivars.Zihuabai exhibited higher activities of SOD and POD in NaCl treated plantlets,and more tolerant to salt stress than Jingshi-2 under in vitro conditions,suggesting its possible utilization in the selection of salinity tolerant genotypes in the field conditions. |
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| Keywords: | MDA POD SOD chlorophyll malondialdehyde (MDA) peroxidase (POD) proline salinity Solanum tuberosum L superoxide dismutase (SOD) |
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