| 贵州多花木兰根际溶磷菌株的鉴定及代谢组学分析 |
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| 引用本文： | 舒健虹,王子苑,欧二绫,李 安,曾庆飞,王小利.贵州多花木兰根际溶磷菌株的鉴定及代谢组学分析[J].西北农业学报,2023(8):1256-1267. |
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| 作者姓名： | 舒健虹 王子苑 欧二绫 李 安 曾庆飞 王小利 |
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| 作者单位： | （1. 贵州省农业科学院 草业研究所，贵阳 550006；2.贵州大学 生命科学学院/农业生物工程研究院，山地植物资源保护与种质创新教育部重点实验室，山地生态与农业生物工程协同创新中心，贵阳 550025） |
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| 基金项目： | 贵州省科技支撑计划(［2019］2359)；贵州省高层次创新人才(［2019］5005)；贵州省农业科技支撑计划（［2019］2365）。 |
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| 摘 要： | 为了研究溶磷菌如何响应和应对缺磷，将难溶性磷转变成可溶性磷的机制。利用贵州多花木兰根际土壤分离筛选获得溶磷菌株PD19-4，设置无磷（CK）、难溶性磷（UP）和可溶性磷（SP）3种磷元素条件下培养，并采用LC-MS/MS 代谢组学方法分析其在不同磷元素条件下培养产生的差异代谢物。经形态学鉴定、理化特性比较和16S rDNA序列比对NCBI数据库，发现PD19-4与巴氏假单胞菌（Pseudomonas baetica）最相似，且同源性达99.65%。代谢组分析发现：（1）菌株代谢物共鉴定出253个。CU（CK∶UP）、CS(CK∶SP)和US(UP∶SP)中差异显著下调的代谢物有68、101和108个。代谢物中有机酸类、氨基酸类、碳水化合物和核苷酸类差异物最多；（2）与CK相比，UP和SP中共同上调较明显的代谢物有磷酸糖类D-甘露糖6-磷酸、D-木酮糖-5-磷酸和核酮糖-5-磷酸类物质，且仅在UP中最活跃的代谢物是D-葡萄糖-6-磷酸和2-脱氧核糖1-磷酸。与UP相比，CK和SP中有机酸类精氨基琥珀酸差异倍数较大且相反，分别为17.28 log2FC和-17.28 log2FC；（3）KEGG注释和富集，与SP相比，CK和UP都共同富集在精氨酸、脯氨酸代谢、缬氨酸、亮氨酸和异亮氨酸代谢途径、碳代谢、磷酸戊糖途径及ABC转运体通路。仅在US中富集的代谢通路有嘌呤代谢、氧化磷酸化、氨基糖和核苷酸糖代谢。综合以上，溶磷菌株从可溶性磷转到难溶性磷环境中，主要通过增加有机酸类和氨基酸类代谢物，同时增强嘌呤代谢、氧化磷酸化、氨基糖和核苷酸糖代谢及磷酸戊糖途径来提高溶磷性。
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| 关 键 词： | 溶磷菌 代谢组学 贵州多花木兰 差异代谢物 |
Identification and Metabonomics Analysis of Phosphorus-soluble Strains in Rhizosphere of Indigofera amblyantha from Guizhou |
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| SHU Jianhong,WANG Ziyuan,OU Erling,LI An,ZEN Qingfei and WANG Xiaoli.Identification and Metabonomics Analysis of Phosphorus-soluble Strains in Rhizosphere of Indigofera amblyantha from Guizhou[J].Acta Agriculturae Boreali-occidentalis Sinica,2023(8):1256-1267. |
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| Authors: | SHU Jianhong WANG Ziyuan OU Erling LI An ZEN Qingfei and WANG Xiaoli |
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| Abstract: | Phosphorus in the soil predominantly exists in the form of insoluble compounds,which lowers the availability of phosphorus and consequently limits the growth of many plants. Phosphate solubilizing bacteria can respond and help ameliorate phosphorus deficiency,but the conversion of insoluble phosphorus into soluble form is poorly understood. In this study,we collected rhizosphere soil of Indigofera amblyantha in Guizhou to isolate and screen the phosphorus-dissolving strain PD19-4,and then analyze the differential metabolites produced under various phosphorus conditions by LC-MS/MS metabolomics methods,so as to understand the metabolic pathways of phosphorus-dissolving strains. Through morphological identification,physical and chemical properties comparison and 16S rDNA sequence blast NCBI,we found that PD19-4 and Pseudomonas baetica had the greatest resemblance and homology reached 99.65%. After metabolome analysis,the results showed that:(1) A total of 253 metabolites were identified. CU(CK:UP),CS(CK:SP) and US(UP:SP) had 68 101 and 108 metabolites,respectively,which were significantly down-regulated. The variants were found mostly in organic acids,amino acids,carbohydrates and nucleotides among all metabolites;(2) Compared with CK,the common up-regulated metabolites in UP and SP wer phosphate sugars D-mannose-6-phosphate,D-xylulose-5-phosphate and ribulose-5-phosphate. And the most active metabolites only observed in UP were D-glucose-6-phosphate and 2-deoxyribose 1-phosphate. Compared with UP,a typical organic acid-argininosuccinic acid showed a higher degree of variance-being surprisingly opposite in CK and SP,which was 17.28 log2FC and -17.28 log2FC,respectively.(3) CK and UP were both enriched in arginine,proline metabolism,valine,leucine and isoleucine metabolic pathways,carbon metabolism,phosphoric acid pentose pathway and ABC transporter pathway compared with SP. The only metabolic pathways enriched in US were purine metabolism,oxidative phosphorylation,amino sugar and nucleotide sugar metabolism. In conclusion,phosphorus-dissolving strains transferred from soluble phosphorus to insoluble phosphorus environment improve phosphorus solubility mainly by increasing the metabolites of organic acids and amino acids,and by enhancing purine metabolism,oxidative phosphorylation,amino sugar and nucleotide sugar metabolism,and phosphopentose pathway. |
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| Keywords: | Phospholytic bacteria Metabolomics Indigofera amblyantha in Guizhou Differential metabolites |
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