| HPLC法测定不同品种鸡蛋中VA和VE含量 |
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| 引用本文： | 杨琴,陈霞,戴瑞彤.HPLC法测定不同品种鸡蛋中VA和VE含量[J].农产品加工．学刊,2007(2):58-61,64. |
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| 作者姓名： | 杨琴 陈霞 戴瑞彤 |
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| 作者单位： | 中国农业大学,食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 采用高效液相色谱法同时测定鸡蛋中的VA和VE含量,样品经皂化后,用石油醚—乙醚萃取,浓缩挥干,用异丙醇溶解后进样。以C18为固定相,甲醇—水(93∶7)为流动相,流速1.0mL/min,采用双波长检测,分别为325nm处测定VA,290nm处测定VE。结果VA含量为0.00151～0.08300g/L,与色谱峰面积呈良好的线性关系;VE含量为0.00531～0.29200g/L,与峰面积呈线性关系。采用HPLC法使鸡蛋中的VA和VE含量测定快速,灵敏,简便易行。
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| 关 键 词： | 胆固醇 VA VE 高效液相色谱 |
| 文章编号： | 1671-9646（2007）02-0058-04 |
| 收稿时间： | 2007-01-25 |
| 修稿时间： | 2007年1月25日 |
Determination of Vitamin A and Vitamin E Contents in Different Egg Products by HPLC Method |
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| Yang Qin,Chen Xia,Dai Ruitong.Determination of Vitamin A and Vitamin E Contents in Different Egg Products by HPLC Method[J].Nongchanpin Jlagong.Xuekan,2007(2):58-61,64. |
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| Authors: | Yang Qin Chen Xia Dai Ruitong |
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| Abstract: | A method for the simultaneous determination of vitamin A and E in egg yolk by high performance liquid chromatography was developed. The sample was determined after being saponified,abstracted with petroleum ether and solved by isopropanol. The C18 column(5μm,250mm×4.6mm)was selected and the mobile phase consisted of carbinol-water (93∶7)at a flow rate of 1.0mL/min. The detection wavelength was 325nm for vitamin A and 290nm for vitamin E. The linear range of vitamin A was between the concentration of 0.001 51 and 0.083g/L;the linear range of vitamin E was between the concentration of 0.005 31 and 0.292g/L. This method is rapid and sensitive,accurate and feasible. |
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| Keywords: | VA VE |
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