Molecular characterization of taro (Colocasia esculenta) using RAPD markers |
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| Authors: | SV Irwin P Kaufusi K Banks R de la Peña JJ Cho |
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| Institution: | (1) Department of Plant Pathology, University of Hawaii, Maui Agricultural Research Center, Kula, HI, 96790, U.S.A;(2) Department of Agronomy and Soils, University of Hawaii, Kauai Agricultural Research Center, Kapaa, HI, 96746, U.S.A |
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| Abstract: | Forty-four taro (Colocasia esculenta), two tanier (Xanthosoma species) and one Colocasia gigantea accessions were evaluated
for genetic diversity using random amplified polymorphic DNA (RAPD) primers. Seventy-three of 112 primers amplified PCR DNA
products used to fingerprint the accessions. Thirty-two primers were considered highly informative because they amplified
more than 5 bands or amplified one or more polymorphic bands that distinguished between accessions. RAPDs showed high genetic
diversity in taro accessions from Indonesia, were capable in distinguishing between Hawaiian accessions, and could separate
triploid from diploid accessions. UPGMA cluster analysis of genetic similarity estimates (Jaccard's coefficient), separated
the accessions into 3 main groups with C. esculenta divided into 5 subgroups. These primers will be useful for future genetic
analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers
identified in the DNA fingerprinting study will be useful to screen a segregating population which is being generated in our
laboratory aimed at developing a taro genetic linkage map.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | taro Colocasia esculenta RAPD DNA fingerprinting phylogenetic tree |
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