| 内蒙古地区3个马品种ELA-DRA* exon2的PCR-SSCP分析 |
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| 引用本文： | 孟青龙,李金莲,石有斐,孙玉江,芒来.内蒙古地区3个马品种ELA-DRA* exon2的PCR-SSCP分析[J].华北农学报,2006,21(5):42-44. |
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| 作者姓名： | 孟青龙 李金莲 石有斐 孙玉江 芒来 |
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| 作者单位： | 1. 内蒙古农业大学,动物科学与医学学院,内蒙,呼和浩特,010018
2. 内蒙古农业大学,动物科学与医学学院,内蒙,呼和浩特,010018;山东省东营市农业科学研究所,山东,东营,257091 |
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| 摘 要： | 为了检测内蒙古地区3个马品种ELA_DRA*exon2的多态性,通过PCR_SSCP技术内蒙古地区3个马品种(三河马、锡尼河马和巴尔虎马)各50匹的ELA_DRA*exon2进行检测,用12%非变性聚丙烯酰胺凝胶电泳将已变性的PCR扩增产物分离,并用银染法显色。结果表明,150匹马中共出现6种基因型:3种纯合子分别记为AA,BB和CC型,均为3条带;3种杂合子分别记为AB,BC和AC型,均为4条带。其中A等位基因频率最高,B次之,而C只在三河马中出现。因此说三河马ELA_DRA*exon2的多态性比锡尼河马和巴尔虎马丰富。
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| 关 键 词： | 马 ELA-DRA exon2 PCR-SSCP 多态性 |
| 文章编号： | 1000-7091（2006）05-0042-03 |
| 收稿时间： | 2005-09-01 |
| 修稿时间： | 2005-09-01 |
Analyzed ELA-DRA* exon2 of Three Horse Breeds from Inner Mongolia by PCR-SSCP |
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| MENG Qing-long,LI Jin-lian,SHI You-fei,SUN Yu-jiang,MANG Lai.Analyzed ELA-DRA* exon2 of Three Horse Breeds from Inner Mongolia by PCR-SSCP[J].Acta Agriculturae Boreali-Sinica,2006,21(5):42-44. |
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| Authors: | MENG Qing-long LI Jin-lian SHI You-fei SUN Yu-jiang MANG Lai |
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| Institution: | 1.College of Animal Science and Veterinary Inner Mongolia Agricultural University,Huhhot 010018;China; 2. Research Institute of Agricultural Science of Dongying Shandong, Dongying 257091, China |
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| Abstract: | In order to detected the polymorphism of three horse breeds in Inner Mongolian region,ELA-DRA~*exon2 locus was used to detect 150 horses of 3 horse breeds from Inner Mongolia,namely Sanhe,Xinihe and Baerhu.The PCR amplified products of the locus were detected by 12 percent non-denatured polyacry lamide gel electrophoresis and showing in silver staining protocol.The results showed that 6 genotypes were detected in 150 horses: 3 homozygotes named AA,BB and CC,respectively,and they all had 3 bands.3 heterozygotes named AB,BC and AC,respectively,and they all had 4 bands.Allele frequency of A were the highest in 3 horse breeds.B were located middle and C can be detected only in Sanhe.So Sanhe had abundant polymorphism than Xinihe and Baerhu in ELA-DRA~*exon2 locus. |
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| Keywords: | Horse ELA-DRA exon2 PCR-SSCP Polymorphism |
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