| 黄瓜白粉病的生防细菌筛选及鉴定 |
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| 引用本文： | 李卫利,何鹏飞,吴毅歆,祁 超,何月秋.黄瓜白粉病的生防细菌筛选及鉴定[J].中国农学通报,2015,31(16):160-165. |
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| 作者姓名： | 李卫利 何鹏飞 吴毅歆 祁 超 何月秋 |
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| 作者单位： | (;1.云南农业大学农业生物多样性应用技术国家工程研究中心,昆明 650201；;2.微生物菌种筛选与应用国家地方联合工程研究中心,昆明 650217；;3.云南农业大学农学与生物技术学院,昆明 650201） |
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| 基金项目： | 基金项目：国际科技合作项目“防治植物病害和促进植物生长的微生物开发应用及机制研究”(2009DFA32360)。 |
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| 摘 要： | 为开发黄瓜白粉病的生防资源，与三七根腐病菌、禾谷镰刀菌和柑橘炭疽病菌进行平板对峙培养，从25株黄瓜根际土细菌和91株烟草内生细菌中筛选出具拮抗功能的菌株；以盆栽试验从拮抗功能菌株中筛选能够防治黄瓜白粉病的生防菌株；用PCR扩增生防菌株的gyrB基因，结合菌株的菌落形态和部分生理生化特征，确定生防菌株分类地位。结果表明，在116株对峙培养细菌中，18株对三七根腐病菌、禾谷镰刀菌和柑橘炭疽病菌有较好拮抗效果。在盆栽试验中，烟草内生菌YN-201490和黄瓜根际菌株WL-1对黄瓜白粉病防效较好，其中YN-201490的1.81×108 cfu/mL和WL-1的1.6×108 cfu/mL稀释液连续施用3次后的防效分别达64.86%和61.26%。最终YN-201490被鉴定为枯草芽孢杆菌，WL-1被鉴定为解淀粉芽孢杆菌。
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| 关 键 词： | 重庆地区 重庆地区 玉米 气候年型 适宜性评价 |
| 收稿时间： | 2015/1/15 0:00:00 |
| 修稿时间： | 2015/5/22 0:00:00 |
Screening and Identification of Bacterial Biocontrol Agents Controlling Cucumber Powdery Mildew |
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| Li Weili,He Pengfei,Wu Yixin,Qi Chao and He Yueqiu.Screening and Identification of Bacterial Biocontrol Agents Controlling Cucumber Powdery Mildew[J].Chinese Agricultural Science Bulletin,2015,31(16):160-165. |
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| Authors: | Li Weili He Pengfei Wu Yixin Qi Chao and He Yueqiu |
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| Institution: | (;1.National Engineering Center for Applied Techniques of Agricultural Biodiversity, Yunnan Agricultural University, Kunming 650201;2.National and Local Joint Engineering Research Center for Microbial Screening and Application, Kunming 650217;3.Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201) |
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| Abstract: | In order to utilize bioagent sources to control cucumber powdery mildew, 25 strains from cucumber rhizospheric bacteria and 91 endophytic bacterial strains of tobacco were used to screen antagonistic bioagents by dual culture experiment with the indicator pathogens Fusarium solani, F. graminearum and Colletotrichum gloeosporioides. The bioagents controlling cucumber powdery mildew were searched from the antagonistic bioagents through pot culture test. The effective bioagents were identified based on colony phenotype, physiological and biochemical characteristics and gyrB gene sequence by PCR. The results showed that 18 bioagents were screened from the 116 bacterial strains and could inhibit the indicators effectively. The endophytic YN-201490 and rhizospheric WL-1 could control the disease with efficiency of 64.86% and 61.26% in the pot culture test when they were sprayed three times with 1.81×108 cfu/mL. YN-201490 and WL-1 were identified as Bacillus subtilis and B. amyloliquefaciens, respectively. |
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| Keywords: | Sphaerotheca fuliginea biological control endophytic bacteria gyrB gene |
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