| 油菜花多糖提取工艺优化研究 |
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| 引用本文： | 孙 茜,夏 琪,易三军,焦 菲,鲁晓晓,唐红枫.油菜花多糖提取工艺优化研究[J].中国农学通报,2015,31(16):267-270. |
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| 作者姓名： | 孙 茜 夏 琪 易三军 焦 菲 鲁晓晓 唐红枫 |
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| 作者单位： | (;1.湖北省疾病预防控制中心,武汉 430079；;2.武汉东湖学院生命科学与化学学院,武汉 430212） |
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| 基金项目： | 基金项目：武汉东湖学院青年基金研究项目“重金属胁迫对蔬菜DNA的损伤及检测指标的建立”。 |
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| 摘 要： | 为提高油菜花多糖的应用价值，以十字花科芸苔属植物油菜花朵为研究对象，研究了油菜花多糖提取优化工艺。油菜花干燥、粉碎，过100目筛，脱脂，通过热水浸提法并结合浸提时间、浸提温度、浸提料水比以及浸提次数等单因素条件进行实验，在此基础上进行正交设计，获得油菜花多糖提取工艺优化条件。实验结果表明，单因素最佳条件下，油菜花多糖得率最大为17.85%；正交优化最佳条件下，油菜花多糖得率为19.18%。正交实验最佳条件为油菜花多糖优化提取工艺，即提取条件为：浸提温度100℃，浸提时间1.5 h，浸提料水比1:40(m:v)，提取次数为2次。
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| 关 键 词： | 赤霉酸 赤霉酸 梨 土壤 消解动态 高效液相色谱 |
| 收稿时间： | 2015/1/26 0:00:00 |
| 修稿时间： | 2015/4/14 0:00:00 |
Optimization Research on Polysaccharides Extraction Process from Brassica chinensis L. |
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| Sun Qian,Xia Qi,Yi Sanjun,Jiao Fei,Lu Xiaoxiao and Tang Hongfeng.Optimization Research on Polysaccharides Extraction Process from Brassica chinensis L.[J].Chinese Agricultural Science Bulletin,2015,31(16):267-270. |
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| Authors: | Sun Qian Xia Qi Yi Sanjun Jiao Fei Lu Xiaoxiao and Tang Hongfeng |
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| Institution: | (;1.Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079;2.College of Life Science and Chemistry, Wuhan Donghu University, Wuhan 430212) |
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| Abstract: | In order to increase the application value of rape polysaccharide, the optimization of rape polysaccharide extraction process was studied with cruciferous plants of the Brassica chinensis as the research object. The rape was dried, crushed, through 100 mesh sieve and degreased. Rape polysaccharide was extracted by hot water extraction according to the extraction time, extraction temperature, extraction water ratio and times of extraction on condition of single factor experiment. The extraction optimization conditions of rape polysaccharide were obtained by orthogonal experimental design. The yield of rape polysaccharide was 17.85% under the optimum condition for the single factor; the yield of rape polysaccharide was 19.18% under the orthogonal optimization optimal conditions. The optimum extraction conditions of rape polysaccharide showed that the water content was 40 (1 g: 40 mL), the extracting temperature was 100℃, the extracting time was 1.5 h, the number of extraction times was two. |
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| Keywords: | rape polysaccharides extraction identification |
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