Complete Nucleotide Sequence of Ryegrass Mottle Virus : A New Species of the Genus Sobemovirus |
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| Authors: | Feiyun ZHANG Shigemitsu TORIYAMA Mami TAKAHASHI |
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| Institution: | (1) National Institute of Agro-Environmental Sciences, Tsukuba 305–8604, Japan, JP |
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| Abstract: | The genome of Ryegrass mottle virus (RGMoV) comprises 4210 nucleotides. The genomic RNA contains four open reading frames (ORFs). The largest ORF 2 encodes a
polyprotein of 947 amino acids (103.6 kDa), which codes for a serine protease and an RNA-dependent RNA polymerase. The viral
coat protein is encoded on ORF 4 present at the 3′-proximal region. Other ORFs 1 and 3 encode the predicted 14.6 kDa and 19.8
kDa proteins of unknown function. The consensus signal for frameshifting, heptanucleotide UUUAAAC and a stem-loop structure
just downstream is in front of the AUG codon of ORF 3. Analysis of the in vitro translation products of RGMoV RNA suggests that the 68 kDa protein may represent a fusion protein of ORF 2-ORF 3 produced
by frameshifting. The protease region of the polyprotein and coat protein have a low similarity with that of the sobemoviruses
(approximately 25% amino acid identity), while the RNA-dependent RNA polymerase region has particularly strong similarity
(54 to 60% of more than 350 amino acid residues). The sequence similarities of RGMoV to the sobemoviruses, together with the
characteristic genome organization indicate that RGMoV is a new species of the genus Sobemovirus.
Received 28 June 2000/ Accepted in revised form 14 November 2000 |
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| Keywords: | : Ryegrass mottle virus nucleotide sequence genome organization sobemovirus |
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