| 生长抑素基因第1外显子多态性与湘西黄牛体尺性状的关联分析 |
| |
| 引用本文： | 罗仍卓么,王兴平,李峰,王京仁,李娜.生长抑素基因第1外显子多态性与湘西黄牛体尺性状的关联分析[J].中国畜牧兽医,2012,39(11):165-168. |
| |
| 作者姓名： | 罗仍卓么 王兴平 李峰 王京仁 李娜 |
| |
| 作者单位： | 1. 湖南文理学院生命科学学院,湖南常德 415000;2. 湖南省高校动物学重点实验室,湖南常德 415000 |
| |
| 基金项目： | 湖南省科技计划项目，湖南省自然科学基金项目，湖南文理学院博士启动项目，湖南省高校创新平台开放基金项目，湖南省动物学重点学科建设项目 |
| |
| 摘 要： | 为探讨湘西黄牛分子遗传特征和寻找生长性状相关的分子标记,本试验采用PCR-SSCP方法检测湘西黄牛生长抑素(somatostatin,SST)基因第1外显子126 bp处g.934G>A位点的多态性,并进行了多态性与生长性状的关联分析。结果表明,湘西黄牛在g.934G>A位点有G和A两个等位基因,G等位基因占优势,检测到GG、AG和AA 3种基因型,呈中度多态,达到Hardy-Weinberg平衡状态(P>0.05)。湘西黄牛GG基因型个体的体高和体长显著大于AA基因型个体(P<0.05);GG和AG基因型个体的胸围和体重极显著大于AA基因型个体(P<0.01)。G等位基因对湘西黄牛的体高、体长、胸围和体重4个性状均为正效应。本试验结果提示,SST基因g.934G>A位点可能为湘西黄牛生长性状的分子遗传标记位点。
|
| 关 键 词： | 湘西黄牛 SST基因 多态性 PCR-SSCP 生长性状 |
| 收稿时间： | 2012-04-06 |
Polymorphisms of Exon 1 of SST Gene and its Associations with Growth Traits in Xiangxi Cattle |
| |
| LUOREN Zhuo-ma
,
WANG Xing-ping
,
LI Feng
,
WANG Jin-ren
,
LI Na.Polymorphisms of Exon 1 of SST Gene and its Associations with Growth Traits in Xiangxi Cattle[J].China Animal Husbandry & Veterinary Medicine,2012,39(11):165-168. |
| |
| Authors: | LUOREN Zhuo-ma WANG Xing-ping LI Feng WANG Jin-ren LI Na |
| |
| Institution: | 1. College of Life Science, Hunan University of Arts and Science, Changde 415000,China;2. Key Laboratory of Zoology in Hunan Higher Education, Changde 415000,China |
| |
| Abstract: | In order to study molecular genetic characters of Xiangxi cattle and search molecular markers related growth traits of Xiangxi cattle, the polymorphism of bovine SST gene was detected by PCR-SSCP, and association between polymorphism of g.934G>A locus in exon 1 of somatostatin (SST) gene and growth traits of Xiangxi cattle were analyzed. The results showed that there were two alleles (G and A) and three genotypes (GG, AG and AA) on g.934G>A locus of SST gene in Xiangxi cattle, which G allele frequency was more than that of A allele. The locus of g.934G>A was in moderate polymorphism, and fitted in Hardy-Weinberg equilibrium (P>0.05). The individuals with GG genotype had larger withers heigh and body length than AA genotype (P<0.05), and the heart girth and body weight of individuals with GG and AG genotypes were significantly larger than AA genotype(P<0.01). G allele had positively effect on withers heigh, body length, heart girth and body weight of Xiangxi cattle. The results of this study suggested that g.934G>A of exon 1 in SST gene might be effect on growth traits of Xiangxi cattle, and it might be used as the molecular genetic marker for breeding. |
| |
| Keywords: | Xiangxi cattle SST gene polymorphism PCR-SSCP growth traits |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
