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| 冷冻速率和解冻温度对猪精液冷冻效果的影响 | |
| 引用本文： | 梁鸿斌,姚学军,李维华,邵慧,朱金清,邬纯鸿,倪和民,刘云海,郭勇.冷冻速率和解冻温度对猪精液冷冻效果的影响[J].中国畜牧兽医,2012,39(11):157-160. |
| 作者姓名： | 梁鸿斌 姚学军 李维华 邵慧 朱金清 邬纯鸿 倪和民 刘云海 郭勇 |
| 作者单位： | 1. 北京奶牛中心,北京,100192 2. 北京市昌平区动物疫病预防控制中心,北京,102200 3. 北京市昌平区种猪场,北京,102212 4. 北京农学院动物科学技术学院,北京,102206 |
| 基金项目： | 十一五国家科技支撑计划项目"荣昌猪资源保护体系及关键技术研究" |
| 摘 要： | 为优化冷冻和解冻方法,提高冷冻效果,本试验比较了不同冷冻速率(-100 ℃ 10 min、-120 ℃ 10 min、-140 ℃ 10 min)和不同解冻温度(37 ℃ 30 s、45 ℃ 30 s、52 ℃ 30 s、60 ℃ 30 s)对猪精液冷冻效果的影响。结果表明,采用-120 ℃熏蒸10 min,解冻后精子活力为0.36,质膜完整率和顶体完整率也优于其他2组,且差异显著(P<0.05)。采用37 ℃ 30 s方法解冻,精子活力、质膜完整率显著高于其他3组,顶体完整率也高于其他3组,但差异不显著(P>0.05),其畸形率最低和60 ℃ 30 s组差异明显(P<0.05),但与45 ℃ 30 s组和52 ℃ 30 s组差异不显著(P>0.05)。因此,采用-120 ℃平衡10 min冷冻,37 ℃ 30 s水浴解冻方法更为适合0.25 mL细管猪冻精解冻。 |
| 关 键 词： | 猪 精液冷冻 细管 冷冻速率 体外受精 |
| 收稿时间： | 2012-05-04 |
Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen |
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| LIANG Hong-bin , YAO Xue-jun , LI Wei-hua , SHAO Hui , ZHU Jin-qing , WU Chun-hong , NI He-min , LIU Yun-hai , GUO Yong.Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen[J].China Animal Husbandry & Veterinary Medicine,2012,39(11):157-160. | |
| Authors: | LIANG Hong-bin YAO Xue-jun LI Wei-hua SHAO Hui ZHU Jin-qing WU Chun-hong NI He-min LIU Yun-hai GUO Yong |
| Institution: | 1. Beijing Dairy Cattle Center,Beijing 100192,China;2. College of Animal Science and Technology, Beijing University of Agriculture,Beijing 102206,China;3. Beijing City of Changping Pig Breeding Farm,Beijing 102212,China;4. Beijing City Changping District Animal Disease Prevention and Control Center,Beijing 102200,China |
| Abstract: | For improving the freezing and de-freezing methods for boar semen further, it was aimed to investigate the effect of different freezing frequency(-100 ℃ 10 min,-120 ℃ 10 min,-140 ℃ 10 min) and thawing temperature(37 ℃ 30 s,45 ℃ 30 s,52 ℃ 30 s,60 ℃ 30 s) on cryopreservation of boar semen. The results showed that the sperm motility, plasma integrity, normal acrosome rate by freezing treatment with -120 ℃ 10 min, were all significantly higher than those of the other three groups respectively(P＜0.05). The sperm motility, plasma integrity, normal acrosome rate by thawing treatment with 37 ℃ 30 s, were not significantly higher than those of the other three groups respectively(P＞0.05), but the difference of the sperm abnormality in the treatment with 60 ℃ 30 s was significant(P＜0.05), but with 45 ℃ 30 s and 52 ℃ 30 s were not significant(P＞0.05). Therefore, it could be concluded that the combination of freezing by -120 ℃ 10 min and thawing by 37 ℃ 30 s were appropriate to frozen-thawed 0.25 mL pipettes of boar semen. |
| Keywords: | boar cryopreservation dtraw freezing frequency artificial insemination |
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