| 副猪嗜血杆菌的分离鉴定及药敏试验 |
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| 引用本文： | 迟阳,高慎阳,郭伶,王宏军,董筱萍,周铁忠,胡桂学.副猪嗜血杆菌的分离鉴定及药敏试验[J].中国畜牧兽医,2012,39(11):206-209. |
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| 作者姓名： | 迟阳 高慎阳 郭伶 王宏军 董筱萍 周铁忠 胡桂学 |
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| 作者单位： | 1. 吉林农业大学动物科学技术学院,吉林长春 130118;2. 辽宁医学院畜牧兽医学院,辽宁锦州 121001 |
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| 基金项目： | 辽宁省教育厅科学研究基金项目，辽宁省农业科技攻关重点项目，辽宁省"百千万人才工程"培养经费资助项目 |
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| 摘 要： | 2011年从辽宁省采集以多发性纤维素性浆膜炎为主要特征的病猪肺脏、心包液、血液等病料,通过分离培养、生化试验和PCR检测等方法确认分离到1株副猪嗜血杆菌Hps LN111013,同时应用多种药物对该菌株进行了药物敏感性试验及分析;结果表明该菌株在形态、培养特性和生化试验等方面与国内其他地区所分离到的菌株基本一致,纸片法药敏试验证明本菌对氟苯尼考、头孢噻呋、洛美沙星最为敏感。本研究为进一步确认辽宁省副猪嗜血杆菌流行菌株的血清型和基因型,查明其传染来源和遗传变异情况奠定了基础,为制定辽宁省副猪嗜血杆菌病防控措施提供依据。
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| 关 键 词： | 副猪嗜血杆菌 分离鉴定 药敏试验 |
| 收稿时间： | 2012-04-07 |
Isolation, Identification and Drug Sensitivity Test of Isolated Haemophilus parasuis |
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| CHI Yang
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GAO Shen-yang
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GUO Ling
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WANG Hong-jun
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DONG Xiao-ping
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ZHOU Tie-zhong
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HU Gui-xue.Isolation, Identification and Drug Sensitivity Test of Isolated Haemophilus parasuis[J].China Animal Husbandry & Veterinary Medicine,2012,39(11):206-209. |
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| Authors: | CHI Yang GAO Shen-yang GUO Ling WANG Hong-jun DONG Xiao-ping ZHOU Tie-zhong HU Gui-xue |
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| Institution: | 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;2. College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, China |
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| Abstract: | One strain of bacteria was isolated from Liaoning samples of pigs with suspected multiple subserosal inflammation and arthritis in 2011. We used the lungs, pericardial fluid, blood from diseased samples via bacterial isolation and culture techniques, biochemisty tests in addition to PCR identification finally indicated that the bacteria belonged to Hemophilus parasuis. Then drug sensitive test and analysis were performed on the strain named HPSLN111013. The results showed that the strain on some aspects such as morpha, cultural character and biochemisty tests were in accord with separate strains from other areas in China. Then drug sensitive test revealed that the isolate was the most sensitive to florfenicol, ceftiofur and lomefloxacin. These results supplied a foundation for the future research of Hemophilus parasuis’s serological and gene typing. Ascertaining the infection source and genetic variation of Hemophilus parasuis provided a basis of prevention and control measures of this disease in Liaoning province. |
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| Keywords: | Hemophilus parasuis isolation and identification drug sensitivity test |
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