| 培养液体积对猪孤雌胚早期发育的影响 |
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| 引用本文： | 许惠艳,罗龙兴,何若钢,卢晟盛,潘天彪,蓝海恩,黄敏瑞,卢克焕.培养液体积对猪孤雌胚早期发育的影响[J].河北农业大学学报,2008,31(5). |
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| 作者姓名： | 许惠艳 罗龙兴 何若钢 卢晟盛 潘天彪 蓝海恩 黄敏瑞 卢克焕 |
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| 作者单位： | 1. 广西亚热带生物资源保护利用重点实验室,广西,南宁,530004;广西大学,动物科技学院,广西,南宁,530004
2. 广西畜牧研究所,广西,南宁,530001 |
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| 基金项目： | 国家自然科学基金，广西科学基金 |
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| 摘 要： | 为了探讨培养液体积对猪早期孤雌胚发育的影响,旨在优化猪早期胚胎培养体系。试验一、二、三、四分别把30、20、15、10个枚孤雌激活胚放入60,45,30,15,10,5μL(10个胚胎)的培养滴,第48小时观察分裂率,第168小时观察囊胚形成率。结果表明:试验一:30~60μL组囊胚率略高于10~15μL组;试验二:60μL组的囊胚率略高;试验三:各组分裂率和囊胚率差异不显著(P>0.05),但45~60μL组分裂率和囊胚率比其他组略高;试验四:15μL组分裂率高于45μL组(P<0.05),15μL组囊胚率显著高于5μL组(P<0.05),其余各组差异不显著。由此表明:30枚猪孤雌胚时,胚胎数∶培养滴体积=1∶(1~2)较好;15~20枚猪孤雌胚时,胚胎数∶培养滴体积=1∶3较好;10枚猪孤雌胚时,胚胎数∶培养滴体积=1∶1.5较好。
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| 关 键 词： | 猪 卵母细胞 孤雌激活 胚胎培养 |
The effect of culture medium volumes on early parthenogenetic development of porcine oocytes |
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| XU Hui-Yan,LUO Long-xing,HE Ruo-gang,LU Sheng-sheng,PAN Tian-biao,LAN Hai-en,HUANG Min-rui,LU Ke-huan.The effect of culture medium volumes on early parthenogenetic development of porcine oocytes[J].Journal of Agricultural University of Hebei,2008,31(5). |
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| Authors: | XU Hui-Yan LUO Long-xing HE Ruo-gang LU Sheng-sheng PAN Tian-biao LAN Hai-en HUANG Min-rui LU Ke-huan |
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| Abstract: | This study aims to investigate the relationship between culture medium volumes and the in vitro development of parthenogenetic oocytes in pig.Experiment 1,2,3,4: 30,20,15,10 embryos per group were randomly cultured in 60,45,30,15,10,5 μL(10 embryos)of culture medium.The cleavage rates of embryos were recorded at 48 h and the blastocyst rates were recorded at 168h.In the experiment 1: the blastocyst rates of 30-60 μL groups were slightly better than those of 10-15 μL groups.In Experiment 2: the blastocyst rate of 60 μL group was slightly higher than those of other groups.In Experiment 3: the cleavage and blastocyst rates of 45-60 μL group were slightly higher than those of other groups.Experiment 4: the blastocyst rate of 15 μL group was slightly higher than that of 5 μL group.In conclusion,when 30 porcine embryos were cultured,the ratio of embryo/volume 1∶(1～2) is better;when the number of embryos between 15 and 20 were cultured,the ratio of embryo/volume 1∶3 is better;and when 10 embryos were cultured,the ratio of embryo/volume 1∶1.5 is better. |
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| Keywords: | pig oocyte parthenogenetic activation embryo culture |
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