| 石漠化地区生态桑林根际土壤微生物资源多样性研究 |
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| 引用本文： | 段倩倩,杨晓红,黄先智.石漠化地区生态桑林根际土壤微生物资源多样性研究[J].西南农业大学学报,2017,39(7):25-29. |
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| 作者姓名： | 段倩倩 杨晓红 黄先智 |
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| 作者单位： | 1. 南方山地园艺学教育部重点实验室/西南大学 园艺园林学院，重庆 400716; 2. 西南大学 蚕学与系统生物学研究所，重庆 400716 |
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| 基金项目： | 重庆市研究生科研创新项目(CYS2015072)；现代农业产业技术体系专项(CARS-22-ZJ0503) |
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| 摘 要： | 以石漠化地区生态桑林根际土壤微生物为研究对象,对微生物群落多样性进行了16s、ITs测序分析以及土壤微生物测序热图分析.结果表明:石漠化地区原核微生物中优势物种为变形菌门、放线菌门和异常球菌-栖热菌门等细菌;优势真核微生物为子囊菌门、接合菌门和担子菌门真菌.热图分析表明,其中:(1)有益微生物包括变性菌门的硫杆菌属、紫色杆菌属和假单胞菌属细菌,异常球菌-栖热菌门的特吕珀菌属细菌以及接合菌门的被孢霉属真菌;(2)与植物病变、腐化有关的真菌种类为子囊菌;(3)Setomelanomma属真菌的功能尚不明确.
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| 关 键 词： | 石漠化 桑根际土壤 微生物资源 微生物分子生态 |
The Diversity of Microbial Resources in Ecological Mulberry Rhizosphere Soil in a Rocky Desertification Area |
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| DUAN Qian-qian,YANG Xiao-hong,HUANG Xian-zhi.The Diversity of Microbial Resources in Ecological Mulberry Rhizosphere Soil in a Rocky Desertification Area[J].Journal of Southwest Agricultural University,2017,39(7):25-29. |
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| Authors: | DUAN Qian-qian YANG Xiao-hong HUANG Xian-zhi |
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| Abstract: | For scientific usage of scarce land resources and improvement of ecological resistance of mulberry, mulberry rhizosphere soil microbes in a rocky desertification area were investigated. Soil microbial community diversity was analysed by ways of 16S rDNA sequence and ITS sequence and soil microbial sequencing heatmap. The results showed that the dominant prokaryotic microbes in this area were bacteria of Proteobacteria, Actinobacteria and Deinococcus-Thermus, and the dominant eukaryotic microorganisms were fungi of Ascomycota, Zygomycota and Basido-mycota. Heatmap analysis showed that beneficial microorganisms included the bacteria genera Thiobacillus, Janthinobacterium and Pseudomonas of Proteobacteria and Truepera of Deinococcus-Thermus, and the fungus genus Mortierella of Zygomycota. Fungi related to plant diseases and decay were found to belong to Ascomycota. The function of the fungus genus Setomelanomma remained undefined. The complex interaction among these microbial communities will be focused on in our next study in order to support microbial fertilizer design in rocky desertification areas. |
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